Glucose uptake in heterologous expression systems

Eunice E. Lee, Richard C. Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells. Once expression of the alleles has been confirmed by Western blotting, the effect of specific mutations on the regulation of glucose transport can be measured through a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages57-67
Number of pages11
Volume1713
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1713
ISSN (Print)1064-3745

Keywords

  • 2-Deoxyglucose
  • 2DG uptake
  • Glucose uptake
  • GLUT1
  • Mammalian cells
  • Radiolabeled glucose
  • Retroviral transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Lee, E. E., & Wang, R. C. (2018). Glucose uptake in heterologous expression systems. In Methods in Molecular Biology (Vol. 1713, pp. 57-67). (Methods in Molecular Biology; Vol. 1713). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7507-5_5