Glucose uptake in heterologous expression systems

Eunice E. Lee, Richard C. Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells. Once expression of the alleles has been confirmed by Western blotting, the effect of specific mutations on the regulation of glucose transport can be measured through a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages57-67
Number of pages11
Volume1713
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1713
ISSN (Print)1064-3745

Fingerprint

Glucose
Facilitative Glucose Transport Proteins
Western Blotting
Alleles
Mutation

Keywords

  • 2-Deoxyglucose
  • 2DG uptake
  • Glucose uptake
  • GLUT1
  • Mammalian cells
  • Radiolabeled glucose
  • Retroviral transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lee, E. E., & Wang, R. C. (2018). Glucose uptake in heterologous expression systems. In Methods in Molecular Biology (Vol. 1713, pp. 57-67). (Methods in Molecular Biology; Vol. 1713). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7507-5_5

Glucose uptake in heterologous expression systems. / Lee, Eunice E.; Wang, Richard C.

Methods in Molecular Biology. Vol. 1713 Humana Press Inc., 2018. p. 57-67 (Methods in Molecular Biology; Vol. 1713).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, EE & Wang, RC 2018, Glucose uptake in heterologous expression systems. in Methods in Molecular Biology. vol. 1713, Methods in Molecular Biology, vol. 1713, Humana Press Inc., pp. 57-67. https://doi.org/10.1007/978-1-4939-7507-5_5
Lee EE, Wang RC. Glucose uptake in heterologous expression systems. In Methods in Molecular Biology. Vol. 1713. Humana Press Inc. 2018. p. 57-67. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7507-5_5
Lee, Eunice E. ; Wang, Richard C. / Glucose uptake in heterologous expression systems. Methods in Molecular Biology. Vol. 1713 Humana Press Inc., 2018. pp. 57-67 (Methods in Molecular Biology).
@inbook{613541ef102848c794cb9ad4b62f56ec,
title = "Glucose uptake in heterologous expression systems",
abstract = "Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells. Once expression of the alleles has been confirmed by Western blotting, the effect of specific mutations on the regulation of glucose transport can be measured through a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.",
keywords = "2-Deoxyglucose, 2DG uptake, Glucose uptake, GLUT1, Mammalian cells, Radiolabeled glucose, Retroviral transduction",
author = "Lee, {Eunice E.} and Wang, {Richard C.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-7507-5_5",
language = "English (US)",
volume = "1713",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "57--67",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Glucose uptake in heterologous expression systems

AU - Lee, Eunice E.

AU - Wang, Richard C.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells. Once expression of the alleles has been confirmed by Western blotting, the effect of specific mutations on the regulation of glucose transport can be measured through a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.

AB - Understanding the physiological regulation of glucose transport requires the analysis of transporters, like GLUT1, in diverse tissue types. We document the utility of viral vectors for the stable expression of wild-type and modified GLUT1 transporter in different types of mammalian cells. Once expression of the alleles has been confirmed by Western blotting, the effect of specific mutations on the regulation of glucose transport can be measured through a previously described radiolabeled glucose transport assay. Although this discussion is focused on the GLUT1 transporter, these techniques are easily transferrable to other glucose transporters.

KW - 2-Deoxyglucose

KW - 2DG uptake

KW - Glucose uptake

KW - GLUT1

KW - Mammalian cells

KW - Radiolabeled glucose

KW - Retroviral transduction

UR - http://www.scopus.com/inward/record.url?scp=85037563814&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037563814&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-7507-5_5

DO - 10.1007/978-1-4939-7507-5_5

M3 - Chapter

C2 - 29218517

AN - SCOPUS:85037563814

VL - 1713

T3 - Methods in Molecular Biology

SP - 57

EP - 67

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -