Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls

L. S. Honig, D. D. Chambliss, E. H. Bigio, S. L. Carroll, J. L. Elliott

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Objective: To ascertain the specificity of alternatively spliced mRNA variants of the astroglial glutamate transporter EAAT2 for ALS. Background: An important hypothesis for ALS pathogenesis is that motor neuron injury may result from chronically elevated glutamate levels in the CNS. Supporting this idea are reports of decreased glutamate transport in ALS. This in turn has recently been suggested to be due to the presence of aberrant mRNA splice variants for EAAT2 in ALS. Methods: Postmortem human brain tissue was obtained from different brain regions of patients with ALS, normal controls (NC), and patients with AD and Lewy body dementia (LB) - neurodegenerative diseases in which motor neurons are unaffected. Brain RNA was analyzed for EAAT2 isoforms using reverse transcription PCR and cDNA cloning/sequencing methods. Results: Splice variants lacking exons 7 or 9 were present in ALS brain, as previously reported, but were also present in brains from NC, AD, and LB patients. PCR product sequence analyses from non-ALS brain show variant splicing identical to that reported for ALS. Quantitative PCR analysis shows that these isoforms may be somewhat more abundant in ALS than AD, LB, and NC brains. Conclusions: EAAT2 mRNA splice variants are found in the brains of NC and AD patients, as in ALS. The authors cannot exclude the possibility that quantitative changes in variant EAAT2 isoforms might relate directly, or indirectly, to ALS pathology. However, the qualitative presence of these 'abnormal' EAAT2 splice variants does not appear to be sufficient to explain motor neuron degeneration in ALS.

Original languageEnglish (US)
Pages (from-to)1082-1088
Number of pages7
JournalNeurology
Volume55
Issue number8
StatePublished - Oct 24 2000

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Amino Acid Transport System X-AG
Brain
Lewy Body Disease
Motor Neurons
Protein Isoforms
Polymerase Chain Reaction
Messenger RNA
Glutamic Acid
Nerve Degeneration
Neurodegenerative Diseases
Reverse Transcription
Sequence Analysis
Organism Cloning
Exons
Complementary DNA
RNA
Pathology
Wounds and Injuries

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Honig, L. S., Chambliss, D. D., Bigio, E. H., Carroll, S. L., & Elliott, J. L. (2000). Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. Neurology, 55(8), 1082-1088.

Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. / Honig, L. S.; Chambliss, D. D.; Bigio, E. H.; Carroll, S. L.; Elliott, J. L.

In: Neurology, Vol. 55, No. 8, 24.10.2000, p. 1082-1088.

Research output: Contribution to journalArticle

Honig, LS, Chambliss, DD, Bigio, EH, Carroll, SL & Elliott, JL 2000, 'Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls', Neurology, vol. 55, no. 8, pp. 1082-1088.
Honig LS, Chambliss DD, Bigio EH, Carroll SL, Elliott JL. Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. Neurology. 2000 Oct 24;55(8):1082-1088.
Honig, L. S. ; Chambliss, D. D. ; Bigio, E. H. ; Carroll, S. L. ; Elliott, J. L. / Glutamate transporter EAAT2 splice variants occur not only in ALS, but also in AD and controls. In: Neurology. 2000 ; Vol. 55, No. 8. pp. 1082-1088.
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