Glutathione in whole blood: a novel determination using double quantum coherence transfer proton NMR spectroscopy

Ralph P. Mason, Gloria H. Cha, George H. Gorrie, Evelyn E. Babcock, Peter P. Antich

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Double quantum selective coherence transfer proton NMR spectroscopy has been used to observe glutathione in whole blood. The efficient water suppression of this technique avoids the need to resuspend the cells in D2O, hence avoiding equilibrium and kinetic isotope effects. Using this method we estimate the concentration of glutathione in fresh whole rabbit blood at ~1.7 mM.

Original languageEnglish (US)
Pages (from-to)30-34
Number of pages5
JournalFEBS Letters
Volume318
Issue number1
DOIs
StatePublished - Feb 22 1993

Keywords

  • Glutathione
  • Proton NMR
  • Rabbit blood

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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