Abstract
Double quantum selective coherence transfer proton NMR spectroscopy has been used to observe glutathione in whole blood. The efficient water suppression of this technique avoids the need to resuspend the cells in D2O, hence avoiding equilibrium and kinetic isotope effects. Using this method we estimate the concentration of glutathione in fresh whole rabbit blood at ~1.7 mM.
Original language | English (US) |
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Pages (from-to) | 30-34 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 318 |
Issue number | 1 |
DOIs | |
State | Published - Feb 22 1993 |
Keywords
- Glutathione
- Proton NMR
- Rabbit blood
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology