Glycine-mediated inhibitory postsynaptic potentials in the medial pontine reticular formation of the rat in vitro

D. R. Stevens, U. Gerber, R. W. McCarley, R. W. Greene

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Glycinergic neurotransmission was examined in rat medial pontine reticular formation neurons in vitro. Intracellular recordings using glass microelectrodes were made in acutely prepared brainstem slices 400 μm thick. Spontaneous and electrically evoked synaptic activity was blocked by the glycine antagonist strychnine (15 μM) but not by the GABA antagonists bicuculline methiodide (40 μM) or picrotoxin (40 μM). Strychnine-sensitive spontaneous and evoked postsynaptic potentials persisted in the presence of the glutamate antagonist (kynurenate, 1 mM). Whole-cell voltage-clamp recordings were carried out in organotypic cultures of rat brainstem. The reversal potential of synaptic currents and responses to exogenously applied glycine were similar and were sensitive to manipulations of the chloride equilibrium potential. Synaptic activity but not responses to exogenous glycine were blocked by tetrodotoxin (0.3 μM). These results indicate the presence of robust, chloride ion-mediated glycinergic inhibition of medial pontine reticular formation neurons, and suggest that glycinergic neurons play an important role in controlling pontine premotor circuitry.

Original languageEnglish (US)
Pages (from-to)791-796
Number of pages6
JournalNeuroscience
Volume73
Issue number3
DOIs
StatePublished - Aug 1996

Fingerprint

Inhibitory Postsynaptic Potentials
Glycine
Strychnine
Neurons
Brain Stem
Chlorides
Miniature Postsynaptic Potentials
GABA Antagonists
Kynurenic Acid
Picrotoxin
Excitatory Amino Acid Antagonists
Synaptic Potentials
Tetrodotoxin
Microelectrodes
Evoked Potentials
Synaptic Transmission
Glass
Ions
In Vitro Techniques
Pontine Tegmentum

Keywords

  • chloride conductance
  • organotypic
  • REM
  • sleep
  • strychnine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Glycine-mediated inhibitory postsynaptic potentials in the medial pontine reticular formation of the rat in vitro. / Stevens, D. R.; Gerber, U.; McCarley, R. W.; Greene, R. W.

In: Neuroscience, Vol. 73, No. 3, 08.1996, p. 791-796.

Research output: Contribution to journalArticle

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