Glycosylation is important for binding to human calcitonin receptors

Hao H. Ho, Matthew T. Gilbert, Daniel R. Nussenzveig, Marvin C. Gershengorn

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Human calcitonin receptor (hCTR) subtypes contain three or four potential Asn-linked glycosylation sites in their extracellular amino termini. The role of glycosylation in hCTR function has not been identified, but it has been suggested that inhibition of glycosylation does not affect binding or signaling. To determine the role of glycosylation in hCTR biology, we studied the effects of inhibition of glycosylation and of substitution of Asn residues that are potential glycosylation sites. Native and mutated hCTRs were studied after transient expression in monkey kidney COS-1 cells. Tunicamycin, administered as part of a treatment protocol that inhibited glycosylation of all expressed receptors, decreased salmon calcitonin (sCT) binding affinities and signaling potencies at hCTRs with three or four potential glycosylation sites. In hCTR3, which contains three potential glycosylation sites at positions 26, 78, and 83, site-specific substitution of Asn-26 by Ala had no effect on sCT binding affinity or potency, whereas substitution of Asn-78 or Asn-83 lowered sCT affinity and potency. A mutant hCTR3 in which all three Asn residues were substituted with Ala exhibited no high-affinity sCT binding and potencies of several calcitonin analogues that were more than 100-fold lower than that of native hCTR3. Our data show that glycosylation is important for high-affinity binding and potency of calcitonin analogues at hCTRs.

Original languageEnglish (US)
Pages (from-to)1866-1872
Number of pages7
JournalBiochemistry
Volume38
Issue number6
DOIs
StatePublished - Feb 9 1999

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Calcitonin Receptors
Glycosylation
salmon calcitonin
Substitution reactions
Calcitonin
Tunicamycin
COS Cells
Clinical Protocols
Haplorhini

ASJC Scopus subject areas

  • Biochemistry

Cite this

Glycosylation is important for binding to human calcitonin receptors. / Ho, Hao H.; Gilbert, Matthew T.; Nussenzveig, Daniel R.; Gershengorn, Marvin C.

In: Biochemistry, Vol. 38, No. 6, 09.02.1999, p. 1866-1872.

Research output: Contribution to journalArticle

Ho, Hao H. ; Gilbert, Matthew T. ; Nussenzveig, Daniel R. ; Gershengorn, Marvin C. / Glycosylation is important for binding to human calcitonin receptors. In: Biochemistry. 1999 ; Vol. 38, No. 6. pp. 1866-1872.
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