GOAT induced ghrelin acylation regulates hedonic feeding

J. F. Davis, M. Perello, D. L. Choi, I. J. Magrisso, H. Kirchner, P. T. Pfluger, M. Tschoep, J. M. Zigman, S. C. Benoit

Research output: Contribution to journalArticle

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Abstract

Ghrelin is an orexigenic hormone that regulates homeostatic and reward-related feeding behavior. Recent evidence indicates that acylation of ghrelin by the gut enzyme ghrelin O-acyl transferase (GOAT) is necessary to render ghrelin maximally active within its target tissues. Here we tested the hypothesis that GOAT activity modulates food motivation and food hedonics using behavioral pharmacology and mutant mice deficient for GOAT and the ghrelin receptor (GHSR). We evaluated operant responding following pharmacological administration of acyl-ghrelin and assessed the necessity of endogenous GOAT activity for operant responding in GOAT and GHSR-null mice. Hedonic-based feeding behavior also was examined in GOAT-KO and GHSR-null mice using a "Dessert Effect" protocol in which the intake of a palatable high fat diet "dessert" was assessed in calorically-sated mice. Pharmacological administration of acyl-ghrelin augmented operant responding; notably, this effect was dependent on intact GHSR signaling. GOAT-KO mice displayed attenuated operant responding and decreased hedonic feeding relative to controls. These behavioral results correlated with decreased expression of the orexin-1 receptor in reward-related brain regions in GOAT-KO mice. In summary, the ability of ghrelin to stimulate food motivation is dependent on intact GHSR signaling and modified by endogenous GOAT activity. Furthermore, GOAT activity is required for hedonic feeding behavior, an effect potentially mediated by forebrain orexin signaling. These data highlight the significance of the GOAT-ghrelin system for the mediation of food motivation and hedonic feeding.

Original languageEnglish (US)
Pages (from-to)598-604
Number of pages7
JournalHormones and Behavior
Volume62
Issue number5
DOIs
StatePublished - Nov 2012

Fingerprint

Ghrelin
Pleasure
Acylation
Transferases
Feeding Behavior
Motivation
Food
Pharmacology
Reward
Orexin Receptors
Ghrelin Receptor
Aptitude

Keywords

  • Ghrelin
  • GOAT
  • Hedonic feeding
  • Operant responding
  • Orexin
  • Reward

ASJC Scopus subject areas

  • Endocrinology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems

Cite this

Davis, J. F., Perello, M., Choi, D. L., Magrisso, I. J., Kirchner, H., Pfluger, P. T., ... Benoit, S. C. (2012). GOAT induced ghrelin acylation regulates hedonic feeding. Hormones and Behavior, 62(5), 598-604. https://doi.org/10.1016/j.yhbeh.2012.08.009

GOAT induced ghrelin acylation regulates hedonic feeding. / Davis, J. F.; Perello, M.; Choi, D. L.; Magrisso, I. J.; Kirchner, H.; Pfluger, P. T.; Tschoep, M.; Zigman, J. M.; Benoit, S. C.

In: Hormones and Behavior, Vol. 62, No. 5, 11.2012, p. 598-604.

Research output: Contribution to journalArticle

Davis, JF, Perello, M, Choi, DL, Magrisso, IJ, Kirchner, H, Pfluger, PT, Tschoep, M, Zigman, JM & Benoit, SC 2012, 'GOAT induced ghrelin acylation regulates hedonic feeding', Hormones and Behavior, vol. 62, no. 5, pp. 598-604. https://doi.org/10.1016/j.yhbeh.2012.08.009
Davis JF, Perello M, Choi DL, Magrisso IJ, Kirchner H, Pfluger PT et al. GOAT induced ghrelin acylation regulates hedonic feeding. Hormones and Behavior. 2012 Nov;62(5):598-604. https://doi.org/10.1016/j.yhbeh.2012.08.009
Davis, J. F. ; Perello, M. ; Choi, D. L. ; Magrisso, I. J. ; Kirchner, H. ; Pfluger, P. T. ; Tschoep, M. ; Zigman, J. M. ; Benoit, S. C. / GOAT induced ghrelin acylation regulates hedonic feeding. In: Hormones and Behavior. 2012 ; Vol. 62, No. 5. pp. 598-604.
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