Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs

Yasuo Yamazaki, Lucy Palmer, Cyrille Alexandre, Satoshi Kakugawa, Karen Beckett, Isabelle Gaugue, Ruth H. Palmer, Jean Paul Vincent

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

The apical and basolateral membranes of epithelia are insulated from each other, preventing the transfer of extracellular proteins from one side to the other. Thus, a signalling protein produced apically is not expected to reach basolateral receptors. Evidence suggests that Wingless, the main Drosophila Wnt, is secreted apically in the embryonic epidermis. However, in the wing imaginal disc epithelium, Wingless is mostly seen on the basolateral membrane where it spreads from secreting to receiving cells. Here we examine the apico-basal movement of Wingless in Wingless-producing cells of wing imaginal discs. We find that it is presented first on the apical surface before making its way to the basolateral surface, where it is released and allowed to interact with signalling receptors. We show that Wingless transcytosis involves dynamin-dependent endocytosis from the apical surface. Subsequent trafficking from early apical endosomes to the basolateral surface requires Godzilla, a member of the RNF family of membrane-anchored E3 ubiquitin ligases. Without such transport, Wingless signalling is strongly reduced in this tissue.

Original languageEnglish (US)
Pages (from-to)451-457
Number of pages7
JournalNature cell biology
Volume18
Issue number4
DOIs
StatePublished - Mar 30 2016
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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