Going forward with genetics: Recent technological advances and forward genetics in mice

Eva Marie Y Moresco, Xiaohong Li, Bruce Beutler

Research output: Contribution to journalArticle

23 Scopus citations


Forward genetic analysis is an unbiased approach for identifying genes essential to defined biological phenomena. When applied to mice, it is one of the most powerful methods to facilitate understanding of the genetic basis of human biology and disease. The speed at which disease-causing mutations can be identified in mutagenized mice has been markedly increased by recent advances in DNA sequencing technology. Creating and analyzing mutant phenotypes may therefore become rate-limiting in forward genetic experimentation. We review the forward genetic approach and its future in the context of recent technological advances, in particular massively parallel DNA sequencing, induced pluripotent stem cells, and haploid embryonic stem cells.

Original languageEnglish (US)
Pages (from-to)1462-1473
Number of pages12
JournalAmerican Journal of Pathology
Issue number5
Publication statusPublished - May 2013


ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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