Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport

Yanzhuang Wang, Jen Hsuan Wei, Blaine Bisel, Danming Tang, Joachim Seemann

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

The Golgi apparatus in mammalian cells is composed of flattened cisternae that are densely packed to form stacks. We have used the Golgi stacking protein GRASP65 as a tool to modify the stacking state of Golgi cistemae. We established an assay to measure protein transport to the cell surface in post-mitotic cells in which the Golgi was unstacked. Cells with an unstacked Golgi showed a higher transport rate compared to cells with stacked Golgi membranes. Vesicle budding from unstacked cisternae in vitro was significantly increased compared to stacked membranes. These results suggest that Golgi cisternal stacking can directly regulate vesicle formation and thus the rate of protein transport through the Golgi. The results further suggest that at the onset of mitosis, unstacking of cistemae allows extensive and rapid vesiculation of the Golgi in preparation for its subsequent partitioning.

Original languageEnglish (US)
Article numbere1647
JournalPLoS One
Volume3
Issue number2
DOIs
StatePublished - Feb 20 2008

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Coat Protein Complex I
protein transport
Protein Transport
Membranes
Proteins
cells
Assays
Cells
Golgi Apparatus
Golgi apparatus
Mitosis
mitosis
assays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport. / Wang, Yanzhuang; Wei, Jen Hsuan; Bisel, Blaine; Tang, Danming; Seemann, Joachim.

In: PLoS One, Vol. 3, No. 2, e1647, 20.02.2008.

Research output: Contribution to journalArticle

Wang, Yanzhuang ; Wei, Jen Hsuan ; Bisel, Blaine ; Tang, Danming ; Seemann, Joachim. / Golgi cisternal unstacking stimulates COPI vesicle budding and protein transport. In: PLoS One. 2008 ; Vol. 3, No. 2.
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