Golgi Phosphoprotein 3 Regulates the Physical Association of Glycolipid Glycosyltransferases

Fernando M. Ruggiero, Natalia Martínez-Koteski, Gerardo D. Fidelio, Aldo A. Vilcaes, Jose L. Daniotti

Research output: Contribution to journalArticlepeer-review

Abstract

Glycolipid glycosylation is an intricate process that mainly takes place in the Golgi by the complex interplay between glycosyltransferases. Several features such as the organization, stoichiometry and composition of these complexes may modify their sorting properties, sub-Golgi localization, enzymatic activity and in consequence, the pattern of glycosylation at the plasma membrane. In spite of the advance in our comprehension about physiological and pathological cellular states of glycosylation, the molecular basis underlying the metabolism of glycolipids and the players involved in this process remain not fully understood. In the present work, using biochemical and fluorescence microscopy approaches, we demonstrate the existence of a physical association between two ganglioside glycosyltransferases, namely, ST3Gal-II (GD1a synthase) and β3GalT-IV (GM1 synthase) with Golgi phosphoprotein 3 (GOLPH3) in mammalian cultured cells. After GOLPH3 knockdown, the localization of both enzymes was not affected, but the fomation of ST3Gal-II/β3GalT-IV complex was compromised and glycolipid expression pattern changed. Our results suggest a novel control mechanism of glycolipid expression through the regulation of the physical association between glycolipid glycosyltransferases mediated by GOLPH3.

Original languageEnglish (US)
JournalInternational journal of molecular sciences
Volume23
Issue number18
DOIs
StatePublished - Sep 8 2022

Keywords

  • gangliosides
  • glycolipids
  • glycosylation
  • glycosyltransferase complex
  • glycosyltransferases
  • Golgi apparatus
  • GOLPH3
  • ST3Gal-II
  • β3GalT-IV

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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