Gonadal expression of Foxo1, but not Foxo3, is conserved in diverse Mammalian species.

Edward D. Tarnawa, Michael D. Baker, Gina M. Aloisio, Bruce R. Carr, Diego H. Castrillon

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The Foxos are key effectors of the PI3K/Akt signaling pathway and regulate diverse physiologic processes. Two of these factors, Foxo1 and Foxo3, serve specific roles in reproduction in the mouse. Foxo3 is required for suppression of primordial follicle activation in females, while Foxo1 regulates spermatogonial stem cell maintenance in males. In the mouse ovary, Foxo1 is highly expressed in somatic cells (but not in oocytes), suggesting an important functional role for Foxo1 in these cells. Given that invertebrate model species such as Caenorhabditis elegans and Drosophila melanogaster harbor a single ancestral Foxo homolog, these observations suggest that gene duplication conferred a selective advantage by permitting the Foxos to adopt distinct roles in oogenesis and spermatogenesis. Our objective was to determine if the remarkably specific expression patterns of Foxo1 and Foxo3 in mouse gonads (and, by inference, Foxo function) are conserved in diverse mammalian species. Western blotting was used to validate isoform-specific antibodies in rodents, companion animals, farm animals, nonhuman primates, and humans. Following validation of each antibody, immunohistochemistry was performed to ascertain Foxo1 and Foxo3 gonadal expression patterns. While Foxo1 expression in spermatogonia and granulosa cells was conserved in each species evaluated, Foxo3 expression in oocytes was not. Our findings suggest that Foxo3 is not uniquely required for primordial follicle maintenance in nonrodent species and that other Foxos, particularly Foxo1, may contribute to oocyte maintenance in a functionally redundant manner.

Original languageEnglish (US)
Article number103
JournalBiology of Reproduction
Volume88
Issue number4
DOIs
StatePublished - Apr 2013

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Oocytes
Maintenance
Oogenesis
Spermatogonia
Gene Duplication
Antibodies
Granulosa Cells
Pets
Domestic Animals
Caenorhabditis elegans
Gonads
Spermatogenesis
Invertebrates
Drosophila melanogaster
Phosphatidylinositol 3-Kinases
Primates
Reproduction
Ovary
Rodentia
Protein Isoforms

ASJC Scopus subject areas

  • Cell Biology

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Gonadal expression of Foxo1, but not Foxo3, is conserved in diverse Mammalian species. / Tarnawa, Edward D.; Baker, Michael D.; Aloisio, Gina M.; Carr, Bruce R.; Castrillon, Diego H.

In: Biology of Reproduction, Vol. 88, No. 4, 103, 04.2013.

Research output: Contribution to journalArticle

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