Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis

Janine Prange-Kiel, Hubertus Jarry, Michael Schoen, Patrick Kohlmann, Christina Lohse, Lepu Zhou, Gabriele M. Rune

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Spine density in the hippocampus changes during the estrus cycle and is dependent on the activity of local aromatase, the final enzyme in estrogen synthesis. In view of the abundant gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA expres sion in the hippocampus and the direct effect of GnRH on estradiol (E2) synthesis in gonadal cells, we asked whether GnRH serves as a regulator of hippocampal E2 synthesis. In hippocampal cultures, E2 synthesis, spine synapse density, and immunoreactivity of spinophilin, a reliable spine marker, are consistently up-regulated in a dose-dependent manner at low doses of GnRH but decrease at higher doses. GnRH is ineffective in the presence of GnRH antagonists or aromatase inhibitors. Conversely, GnRH-R expression increases after inhibition of hippocampal aromatase. As we found estrus cyclicity of spine density in the hippocampus but not in the neocortex and GnRH-R expression to be five fold higher in the hippocampus compared with the neocortex, our data strongly suggest that estrus cycle-dependent synaptogenesis in the female hippocampus results from cyclic release of GnRH.

Original languageEnglish (US)
Pages (from-to)417-426
Number of pages10
JournalJournal of Cell Biology
Volume180
Issue number2
DOIs
StatePublished - Jan 28 2008

Fingerprint

Gonadotropin-Releasing Hormone
Estrogens
Spine
Hippocampus
LHRH Receptors
Estrus
Aromatase
Neocortex
Aromatase Inhibitors
Periodicity
Synapses
Estradiol
Messenger RNA
Enzymes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis. / Prange-Kiel, Janine; Jarry, Hubertus; Schoen, Michael; Kohlmann, Patrick; Lohse, Christina; Zhou, Lepu; Rune, Gabriele M.

In: Journal of Cell Biology, Vol. 180, No. 2, 28.01.2008, p. 417-426.

Research output: Contribution to journalArticle

Prange-Kiel, Janine ; Jarry, Hubertus ; Schoen, Michael ; Kohlmann, Patrick ; Lohse, Christina ; Zhou, Lepu ; Rune, Gabriele M. / Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis. In: Journal of Cell Biology. 2008 ; Vol. 180, No. 2. pp. 417-426.
@article{8075aa3ca71543f9bbec55b1aaef56a7,
title = "Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis",
abstract = "Spine density in the hippocampus changes during the estrus cycle and is dependent on the activity of local aromatase, the final enzyme in estrogen synthesis. In view of the abundant gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA expres sion in the hippocampus and the direct effect of GnRH on estradiol (E2) synthesis in gonadal cells, we asked whether GnRH serves as a regulator of hippocampal E2 synthesis. In hippocampal cultures, E2 synthesis, spine synapse density, and immunoreactivity of spinophilin, a reliable spine marker, are consistently up-regulated in a dose-dependent manner at low doses of GnRH but decrease at higher doses. GnRH is ineffective in the presence of GnRH antagonists or aromatase inhibitors. Conversely, GnRH-R expression increases after inhibition of hippocampal aromatase. As we found estrus cyclicity of spine density in the hippocampus but not in the neocortex and GnRH-R expression to be five fold higher in the hippocampus compared with the neocortex, our data strongly suggest that estrus cycle-dependent synaptogenesis in the female hippocampus results from cyclic release of GnRH.",
author = "Janine Prange-Kiel and Hubertus Jarry and Michael Schoen and Patrick Kohlmann and Christina Lohse and Lepu Zhou and Rune, {Gabriele M.}",
year = "2008",
month = "1",
day = "28",
doi = "10.1083/jcb.200707043",
language = "English (US)",
volume = "180",
pages = "417--426",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Gonadotropin-releasing hormone regulates spine density via its regulatory role in hippocampal estrogen synthesis

AU - Prange-Kiel, Janine

AU - Jarry, Hubertus

AU - Schoen, Michael

AU - Kohlmann, Patrick

AU - Lohse, Christina

AU - Zhou, Lepu

AU - Rune, Gabriele M.

PY - 2008/1/28

Y1 - 2008/1/28

N2 - Spine density in the hippocampus changes during the estrus cycle and is dependent on the activity of local aromatase, the final enzyme in estrogen synthesis. In view of the abundant gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA expres sion in the hippocampus and the direct effect of GnRH on estradiol (E2) synthesis in gonadal cells, we asked whether GnRH serves as a regulator of hippocampal E2 synthesis. In hippocampal cultures, E2 synthesis, spine synapse density, and immunoreactivity of spinophilin, a reliable spine marker, are consistently up-regulated in a dose-dependent manner at low doses of GnRH but decrease at higher doses. GnRH is ineffective in the presence of GnRH antagonists or aromatase inhibitors. Conversely, GnRH-R expression increases after inhibition of hippocampal aromatase. As we found estrus cyclicity of spine density in the hippocampus but not in the neocortex and GnRH-R expression to be five fold higher in the hippocampus compared with the neocortex, our data strongly suggest that estrus cycle-dependent synaptogenesis in the female hippocampus results from cyclic release of GnRH.

AB - Spine density in the hippocampus changes during the estrus cycle and is dependent on the activity of local aromatase, the final enzyme in estrogen synthesis. In view of the abundant gonadotropin-releasing hormone receptor (GnRH-R) messenger RNA expres sion in the hippocampus and the direct effect of GnRH on estradiol (E2) synthesis in gonadal cells, we asked whether GnRH serves as a regulator of hippocampal E2 synthesis. In hippocampal cultures, E2 synthesis, spine synapse density, and immunoreactivity of spinophilin, a reliable spine marker, are consistently up-regulated in a dose-dependent manner at low doses of GnRH but decrease at higher doses. GnRH is ineffective in the presence of GnRH antagonists or aromatase inhibitors. Conversely, GnRH-R expression increases after inhibition of hippocampal aromatase. As we found estrus cyclicity of spine density in the hippocampus but not in the neocortex and GnRH-R expression to be five fold higher in the hippocampus compared with the neocortex, our data strongly suggest that estrus cycle-dependent synaptogenesis in the female hippocampus results from cyclic release of GnRH.

UR - http://www.scopus.com/inward/record.url?scp=38749095857&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38749095857&partnerID=8YFLogxK

U2 - 10.1083/jcb.200707043

DO - 10.1083/jcb.200707043

M3 - Article

C2 - 18227283

AN - SCOPUS:38749095857

VL - 180

SP - 417

EP - 426

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2

ER -