gp63 homologues in Trypanosoma cruzi

Surface antigens with metalloprotease activity and a possible role in host cell infection

Ileana C. Cuevas, Juan J. Cazzulo, Daniel O. Sánchez

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

gp63 is a highly abundant glycosylphosphatidylinositol (GPI)-anchored membrane protein expressed predominantly in the promastigote but also in the amastigote stage of Leishmania species. In Leishmania spp., gp63 has been implicated in a number of steps in establishment of infection. Here we demonstrate that Trypanosoma cruzi, the etiological agent of Chagas' disease, has a family of gp63 genes composed of multiple groups. Two of these groups, Tcgp63-I and -II, are present as high-copy-number genes. The genomic organization and mRNA expression pattern were specific for each group. Tcgp63-I was widely expressed, while the Tcgp63-H group was scarcely detected in Northern blots, even though it is well represented in the T. cruzi genome. Western blots using sera directed against a synthetic peptide indicated that the Tcgp63-I group produced proteins of ∼78 kDa, differentially expressed during the life cycle. Immunofluorescence staining and phosphatidylinositol-specific phospholipase C digestion confirmed that Tcgp63-I group members are surface proteins bound to the membrane by a GPI anchor. We also demonstrate the presence of metalloprotease activity which is attributable, at least in part, to Tcgp63-I group. Since antibodies against Tcgp63-I partially blocked infection of Vero cells by trypomastigotes, a possible role for this group in infection is suggested.

Original languageEnglish (US)
Pages (from-to)5739-5749
Number of pages11
JournalInfection and Immunity
Volume71
Issue number10
DOIs
StatePublished - Oct 1 2003

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Trypanosoma cruzi
Metalloproteases
Surface Antigens
Glycosylphosphatidylinositols
Membrane Proteins
Infection
Phosphoinositide Phospholipase C
Vero Cells
Gene Dosage
Chagas Disease
Leishmania
Life Cycle Stages
Northern Blotting
Fluorescent Antibody Technique
Digestion
Western Blotting
Genome
Staining and Labeling
Messenger RNA
Peptides

ASJC Scopus subject areas

  • Immunology

Cite this

gp63 homologues in Trypanosoma cruzi : Surface antigens with metalloprotease activity and a possible role in host cell infection. / Cuevas, Ileana C.; Cazzulo, Juan J.; Sánchez, Daniel O.

In: Infection and Immunity, Vol. 71, No. 10, 01.10.2003, p. 5739-5749.

Research output: Contribution to journalArticle

Cuevas, Ileana C. ; Cazzulo, Juan J. ; Sánchez, Daniel O. / gp63 homologues in Trypanosoma cruzi : Surface antigens with metalloprotease activity and a possible role in host cell infection. In: Infection and Immunity. 2003 ; Vol. 71, No. 10. pp. 5739-5749.
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