Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae

David E. Greenberg, Stephen F. Porcella, Frida Stock, Alexandra Wong, Patricia S. Conville, Patrick R. Murray, Steven M. Holland, Adrian M. Zelazny

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

A Gram-negative, aerobic, coccobacillus to rod-shaped bacterium was isolated from three patients with chronic granulomatous disease. The organism was subjected to a polyphasic taxonomic study. A multilocus; phylogenetic analysis based on the 16S rRNA gene, the internal transcribed spacer (ITS) region and the RecA protein demonstrated that the organism belongs to a new sublineage within the acetic acid bacteria in the family Acetobacteraceae. Phenotypic features are summarized as follows: the organism grew at an optimum temperature of 35-37 °C and optimum pH of 5.0-6.5. It produced a yellow pigment, oxidized lactate and acetate, the latter weakly, produced little acetic acid from ethanol and could use methanol as a sole carbon source. The two major fatty acids were a straight-chain unsaturated acid (C18: 1ω7c) and C16: 0. The DNA base composition was 59-1 mol% G + C. The very weak production of acetic acid from ethanol, the ability to use methanol, the yellow pigmentation and high optimum temperature for growth distinguished this organism from other acetic acid bacteria. The unique phylogenetic and phenotypic characteristics suggest that the bacterium should be classified within a separate genus, for which the name Granulibacter bethesdensis gen. nov., sp. nov. is proposed. The type strain is CGDNIH1T(= ATCC BAA-1260T =DSM 17861T).

Original languageEnglish (US)
Pages (from-to)2609-2616
Number of pages8
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number11
DOIs
StatePublished - Nov 2006

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Acetobacteraceae
acetic acid bacteria
Acetic Acid
acetic acid
Bacteria
bacterium
new species
organisms
Methanol
methanol
ethanol
Ethanol
Rec A Recombinases
phylogenetics
Chronic Granulomatous Disease
Temperature
phylogeny
bacteria
Base Composition
Pigmentation

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae. / Greenberg, David E.; Porcella, Stephen F.; Stock, Frida; Wong, Alexandra; Conville, Patricia S.; Murray, Patrick R.; Holland, Steven M.; Zelazny, Adrian M.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 11, 11.2006, p. 2609-2616.

Research output: Contribution to journalArticle

Greenberg, David E. ; Porcella, Stephen F. ; Stock, Frida ; Wong, Alexandra ; Conville, Patricia S. ; Murray, Patrick R. ; Holland, Steven M. ; Zelazny, Adrian M. / Granulibacter bethesdensis gen. nov., sp. nov., a distinctive pathogenic acetic acid bacterium in the family Acetobacteraceae. In: International Journal of Systematic and Evolutionary Microbiology. 2006 ; Vol. 56, No. 11. pp. 2609-2616.
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