Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-α plus fibronectin

S. Esnault, J. S. Malter

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Airway eosinophils show prolonged in vitro survival compared with peripheral blood eosinophils (PBEos). Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-α increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. These data suggest that TNF-α plus fibronectin may increase eosinophil survival in vivo by controlling GM-CSF production at a posttranscriptional level.

Original languageEnglish (US)
Pages (from-to)4658-4663
Number of pages6
JournalJournal of Immunology
Volume166
Issue number7
DOIs
StatePublished - Apr 1 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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