Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-α plus fibronectin

S. Esnault, J. S. Malter

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Airway eosinophils show prolonged in vitro survival compared with peripheral blood eosinophils (PBEos). Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-α increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. These data suggest that TNF-α plus fibronectin may increase eosinophil survival in vivo by controlling GM-CSF production at a posttranscriptional level.

Original languageEnglish (US)
Pages (from-to)4658-4663
Number of pages6
JournalJournal of Immunology
Volume166
Issue number7
DOIs
StatePublished - Apr 1 2001

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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