Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation

Hongtao Wang; Shanhu Li; Hailiang Li; Peng Wang; Fang Huang; Yali Zhao; Lan Yu; Guolan Luo; Xiaoqing Zhang; Jian Wang; Jianguang Zhou

doi:10.1007/s11010-013-1945-7

Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation

Hongtao Wang, Shanhu Li, Hailiang Li, Peng Wang, Fang Huang, Yali Zhao, Lan Yu, Guolan Luo, Xiaoqing Zhang, Jian Wang, Jianguang Zhou

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.

Original language	English (US)
Pages (from-to)	239-247
Number of pages	9
Journal	Molecular and Cellular Biochemistry
Volume	389
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-013-1945-7
State	Published - Apr 2014
Externally published	Yes

Keywords

EGF
EGFR
ERK1/2
Grb2
SGEF

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/s11010-013-1945-7

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title = "Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation",

abstract = "Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.",

keywords = "EGF, EGFR, ERK1/2, Grb2, SGEF",

author = "Hongtao Wang and Shanhu Li and Hailiang Li and Peng Wang and Fang Huang and Yali Zhao and Lan Yu and Guolan Luo and Xiaoqing Zhang and Jian Wang and Jianguang Zhou",

note = "Funding Information: Acknowledgments We thank Dr. Wannian Yang for providing materials. This work was supported by the grants from the National Natural Science Foundation of China (No. 81372770 to J.Z. and Nos. 81172445 and 81372140 to J.W.).",

year = "2014",

month = apr,

doi = "10.1007/s11010-013-1945-7",

language = "English (US)",

volume = "389",

pages = "239--247",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

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TY - JOUR

T1 - Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation

AU - Wang, Hongtao

AU - Li, Shanhu

AU - Li, Hailiang

AU - Wang, Peng

AU - Huang, Fang

AU - Zhao, Yali

AU - Yu, Lan

AU - Luo, Guolan

AU - Zhang, Xiaoqing

AU - Wang, Jian

AU - Zhou, Jianguang

N1 - Funding Information: Acknowledgments We thank Dr. Wannian Yang for providing materials. This work was supported by the grants from the National Natural Science Foundation of China (No. 81372770 to J.Z. and Nos. 81172445 and 81372140 to J.W.).

PY - 2014/4

Y1 - 2014/4

N2 - Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.

AB - Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.

KW - EGF

KW - EGFR

KW - ERK1/2

KW - Grb2

KW - SGEF

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SN - 0300-8177

VL - 389

SP - 239

EP - 247

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation

Abstract

Keywords

ASJC Scopus subject areas

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