Grb2 interacts with SGEF and antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation

Hongtao Wang, Shanhu Li, Hailiang Li, Peng Wang, Fang Huang, Yali Zhao, Lan Yu, Guolan Luo, Xiaoqing Zhang, Jian Wang, Jianguang Zhou

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Previously, we demonstrated that SGEF enhances EGFR stability; however, SGEF-mediated downstream signaling of EGFR is not well understood. Here, we show that SGEF enhances EGF-induced ERK1/2 activation independent of its guanine nucleotide exchange (GEF) activity. We further show that SGEF interacts with Grb2, a critical downstream transducer of EGFR. Surprisingly, we found that interaction of Grb2 to SGEF antagonizes the ability of SGEF to enhance EGF-induced ERK1/2 activation. Taken together, this study reports a novel function of SGEF that excludes GEF and also provides important insights into the complex role of Grb2 in EGFR signal transduction.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume389
Issue number1-2
DOIs
StatePublished - Apr 2014
Externally publishedYes

Keywords

  • EGF
  • EGFR
  • ERK1/2
  • Grb2
  • SGEF

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

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