Greater insulin sensitivity in calorie restricted rats occurs with unaltered circulating levels of several important myokines and cytokines

Naveen Sharma, Carlos M. Castorena, Gregory D. Cartee

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Calorie restriction (CR; ∼60% of ad libitum, AL intake) has been associated with substantial alterations in body composition and insulin sensitivity. Recently, several proteins that are secreted by nontraditional endocrine tissues, including skeletal muscle and other tissues, have been discovered to modulate energy metabolism, body composition, and insulin sensitivity. The aim of this study was to characterize the influence of CR by rats on plasma levels of six of these newly recognized metabolic hormones (BDNF, FGF21, IL-1β, myonectin, myostatin, and irisin). Body composition of 9-month old male Fischer-344/Brown Norway rats (AL and CR groups) was determined by nuclear magnetic resonance. Blood sampled from the carotid artery of unanesthetized rats was used to measure concentrations of glucose and plasma proteins. As expected, CR versus AL rats had significantly altered body composition (reduced percent fat mass, increased percent lean mass) and significantly improved insulin sensitivity (based on the homeostasis model assessment-estimated insulin resistance index). Also consistent with previous reports, CR compared to AL rats had significantly greater plasma levels of adiponectin and corticosterone. However, there were no significant diet-related differences in plasma levels of BDNF, FGF21, IL-1β, myonectin, myostatin, or irisin. In conclusion, these results indicate that alterations in plasma concentration of these six secreted proteins are not essential for the CR-related improvement in insulin sensitivity in rats.

Original languageEnglish (US)
Article number90
JournalNutrition and Metabolism
Volume9
DOIs
StatePublished - 2012

Keywords

  • Adiponectin
  • Dietary restriction
  • FGF21
  • FNDC5
  • Insulin resistance
  • Irisin
  • Myonectin
  • Myostatin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Fingerprint Dive into the research topics of 'Greater insulin sensitivity in calorie restricted rats occurs with unaltered circulating levels of several important myokines and cytokines'. Together they form a unique fingerprint.

  • Cite this