Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

Pin Pin Hsu; Benjamin Chen; Suli Yuan; Konan Peck; John Y J Shyy; Shu Chien

Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

Pin Pin Hsu, Benjamin Chen, Suli Yuan, Konan Peck, John Y J Shyy, Shu Chien

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Laminar shear stress inhibits endothelial cell proliferation in vitro. Using DNA microarray technology, we found that many genes involved in growth arrest were up-regulated by laminar shear stress, including the Growth Arrest and DNA Damage Inducible Gene 45 (GADD45). The mRNA and protein levels of GADD45 were both increased in response to laminar flow, as shown by Northern blot and Western blot, respectively. The induction of GADD45 promoter by laminar shear stress was regulated by the tumor suppressor gene p53. These results provide a molecular basis for the inhibition of endothelial cell proliferation by laminar shear stress.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Publisher	IEEE
Pages	8
Number of pages	1
ISBN (Print)	0780356756
State	Published - 1999
Event	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA Duration: Oct 13 1999 → Oct 16 1999

Publication series

Name	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume	1
ISSN (Print)	0589-1019

Other

Other	Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
City	Atlanta, GA, USA
Period	10/13/99 → 10/16/99

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

Cite this

Hsu, P. P., Chen, B., Yuan, S., Peck, K., Shyy, J. Y. J., & Chien, S. (1999). Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (pp. 8). (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1). IEEE.

Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. / Hsu, Pin Pin; Chen, Benjamin; Yuan, Suli et al.
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. p. 8 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hsu, PP, Chen, B, Yuan, S, Peck, K, Shyy, JYJ & Chien, S 1999, Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 1, IEEE, pp. 8, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.

Hsu PP, Chen B, Yuan S, Peck K, Shyy JYJ, Chien S. Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE. 1999. p. 8. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).

Hsu, Pin Pin ; Chen, Benjamin ; Yuan, Suli et al. / Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. IEEE, 1999. pp. 8 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).

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title = "Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology",

abstract = "Laminar shear stress inhibits endothelial cell proliferation in vitro. Using DNA microarray technology, we found that many genes involved in growth arrest were up-regulated by laminar shear stress, including the Growth Arrest and DNA Damage Inducible Gene 45 (GADD45). The mRNA and protein levels of GADD45 were both increased in response to laminar flow, as shown by Northern blot and Western blot, respectively. The induction of GADD45 promoter by laminar shear stress was regulated by the tumor suppressor gene p53. These results provide a molecular basis for the inhibition of endothelial cell proliferation by laminar shear stress.",

author = "Hsu, {Pin Pin} and Benjamin Chen and Suli Yuan and Konan Peck and Shyy, {John Y J} and Shu Chien",

year = "1999",

language = "English (US)",

isbn = "0780356756",

series = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

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booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

note = "Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) ; Conference date: 13-10-1999 Through 16-10-1999",

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T1 - Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

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AU - Shyy, John Y J

AU - Chien, Shu

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N2 - Laminar shear stress inhibits endothelial cell proliferation in vitro. Using DNA microarray technology, we found that many genes involved in growth arrest were up-regulated by laminar shear stress, including the Growth Arrest and DNA Damage Inducible Gene 45 (GADD45). The mRNA and protein levels of GADD45 were both increased in response to laminar flow, as shown by Northern blot and Western blot, respectively. The induction of GADD45 promoter by laminar shear stress was regulated by the tumor suppressor gene p53. These results provide a molecular basis for the inhibition of endothelial cell proliferation by laminar shear stress.

AB - Laminar shear stress inhibits endothelial cell proliferation in vitro. Using DNA microarray technology, we found that many genes involved in growth arrest were up-regulated by laminar shear stress, including the Growth Arrest and DNA Damage Inducible Gene 45 (GADD45). The mRNA and protein levels of GADD45 were both increased in response to laminar flow, as shown by Northern blot and Western blot, respectively. The induction of GADD45 promoter by laminar shear stress was regulated by the tumor suppressor gene p53. These results provide a molecular basis for the inhibition of endothelial cell proliferation by laminar shear stress.

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Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

Abstract

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