Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology

Pin Pin Hsu, Benjamin Chen, Suli Yuan, Konan Peck, John Y J Shyy, Shu Chien

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Laminar shear stress inhibits endothelial cell proliferation in vitro. Using DNA microarray technology, we found that many genes involved in growth arrest were up-regulated by laminar shear stress, including the Growth Arrest and DNA Damage Inducible Gene 45 (GADD45). The mRNA and protein levels of GADD45 were both increased in response to laminar flow, as shown by Northern blot and Western blot, respectively. The induction of GADD45 promoter by laminar shear stress was regulated by the tumor suppressor gene p53. These results provide a molecular basis for the inhibition of endothelial cell proliferation by laminar shear stress.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Place of PublicationPiscataway, NJ, United States
PublisherIEEE
Pages8
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

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ASJC Scopus subject areas

  • Bioengineering

Cite this

Hsu, P. P., Chen, B., Yuan, S., Peck, K., Shyy, J. Y. J., & Chien, S. (1999). Growth arrest and DNA Damage Inducible Gene 45 (GADD45) is induced by laminar shear stress as identified by using DNA microarray technology. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 8). IEEE.