Growth hormone, growth factors and hematopoiesis

Keith W. Kelley, Sean Arkins, Christian T Minshall, Qiang Liu, Robert Dantzer

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Hypocellularity of primary lymphoid organs is a distinctive and reproducible characteristic of aged humans and animals. Similar changes have been reported in both hypophysectomized and dwarf rodents. In the bone marrow of these animals, there is an associated reduction in the number of erythroid, lymphoid and myeloid elements. Implantation of growth hormone (GH)-secreting GH3 pituitary cells or infusion of growth hormone into aged rodents dramatically improves cellularity of both the thymus gland and bone marrow. At present it is unknown whether these effects are due to direct effects of growth hormone on hematopoietic cells or if they are caused by the induction of insulin-like growth factor-1 (IGF-1) synthesis. We recently discovered that colony-stimulating factor-1 (CSF-1) and interleukin-3 (IL-3) induce expression and synthesis of the IGF-1 peptide in murine bone marrow cells. Transcripts for IGF-1 increase approximately 50-fold during differentiation over the negligible levels that are expressed in freshly isolated bone marrow cells. Two potential functions of macrophage-derived IGF-1 are to: (a) increase the proliferation of early or committed bone marrow progenitors and (b) reduce their rate of cell death. In support of the first possibility, IGF binding protein-3 significantly inhibits the proliferation of CSF-1-treated bone marrow cells and this inhibition can be reversed by addition of exogenous IGF-1. In support of the second possibility, we have induced apoptosis of both nonadherent bone marrow cells and a myeloid progenitor cell line by depriving these cells of CSFs. Preliminary results indicate that addition of IGF-1 to these cells reduces apoptotic cell death by 50%. These data establish that two different CSFs, CSF-1 and IL-3, induce abundant expression of IGF-1 as these cells differentiate into more mature hematopoietic cells. This model offers a novel approach for investigating the developmental expression of IGF-1 during defined differentiation pathways of hematopoietic cells. If IGF-1 is indeed proven to act as a survival factor for hematopoietic progenitors, these data would support the idea that the hypocellularity of primary lymphoid tissues in aged animals is related to the limited availability to these cells of either growth hormone or IGF-1.

Original languageEnglish (US)
Pages (from-to)38-45
Number of pages8
JournalHormone Research
Volume45
Issue number1-2
StatePublished - Jan 1996

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Hematopoiesis
Somatomedins
Growth Hormone
Intercellular Signaling Peptides and Proteins
Bone Marrow Cells
Macrophage Colony-Stimulating Factor
Interleukin-3
Bone Marrow
Rodentia
Cell Death
Somatotrophs
Myeloid Progenitor Cells
Insulin-Like Growth Factor Binding Protein 3
Lymphoid Tissue
Thymus Gland
Macrophages
Apoptosis
Cell Line
Peptides

Keywords

  • Apoptosis
  • Colony-stimulating factors
  • IGF-1
  • Myeloid cells

ASJC Scopus subject areas

  • Endocrinology

Cite this

Kelley, K. W., Arkins, S., Minshall, C. T., Liu, Q., & Dantzer, R. (1996). Growth hormone, growth factors and hematopoiesis. Hormone Research, 45(1-2), 38-45.

Growth hormone, growth factors and hematopoiesis. / Kelley, Keith W.; Arkins, Sean; Minshall, Christian T; Liu, Qiang; Dantzer, Robert.

In: Hormone Research, Vol. 45, No. 1-2, 01.1996, p. 38-45.

Research output: Contribution to journalArticle

Kelley, KW, Arkins, S, Minshall, CT, Liu, Q & Dantzer, R 1996, 'Growth hormone, growth factors and hematopoiesis', Hormone Research, vol. 45, no. 1-2, pp. 38-45.
Kelley KW, Arkins S, Minshall CT, Liu Q, Dantzer R. Growth hormone, growth factors and hematopoiesis. Hormone Research. 1996 Jan;45(1-2):38-45.
Kelley, Keith W. ; Arkins, Sean ; Minshall, Christian T ; Liu, Qiang ; Dantzer, Robert. / Growth hormone, growth factors and hematopoiesis. In: Hormone Research. 1996 ; Vol. 45, No. 1-2. pp. 38-45.
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