Growth hormone stimulates bone marrow and thymic reconstitution in aged rats

William A. Meier, Christian T Minshall, Sean Arkins, Richard A. French, James F. Zachary, Keith W. Kelley

Research output: Contribution to journalArticle

Abstract

The age-associated dysregulation in immunologie function is related to a decline in plasma growth hormone (GH) and its growth-promoting peptide insulin-like growth factor-I (IGF-I). We recently demonstrated that IGF-I inhibits apoptosis in murine myeloid progenitor cells in vitro (J. Immunol. 156:939, 1996), suggesting that GH or IGF-I may play a role in maintaining hematopoietic tissues in the aged. In the present study, GH-secreting pituitary GH, cells were implanted into 22-month-old female Wistar-Furth rats for eight weeks. We compared tissues from GH, implanted, rHuGH ( 1 mg/kg, bidx28d) and saline-injected control rats. Gross and microscopic observations in aged rats treated with GH, cells or rHuGH included: reconstitution of bone marrow celluiarity, thymic regeneration and extramedullary hematopoiesis with marked splenomegaly. In aged GH,-and rHuGH-treated rats, bone marrow celluiarity increased (400+33% and 200+8%, respectively, p<0.01) as compared to aged controls. The increase in marrow celluiarity was primarily attributed to erythroid and myeloid proliferation, but all lineages were represented. Using an in vitro colony forming assay (250 U/ml rMuIL-3 + 1.25 ng/ml rMuGM-CSF), we found that the number of myeloid progenitors was reduced in aged rats as compared to young controls (54+4 vs 81+3/2xl04 cells, respectively; p<0.01). Treatment of aged rats with either GH, cells or rHuGH effectively reversed the age-associated decline in myeloid progenitors (144+13% and 145+15% compared to aged rats, respectively; p<0.01). These results suggest that GH acts in vivo to promote myeloid, erythroid and lymphoid differentiation and proliferation in aged rats.(NIH Grant AG 06246).

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

bone marrow
somatotropin
Growth Hormone
Rats
Bone
Bone Marrow
rats
Insulin-Like Growth Factor I
insulin-like growth factor I
Inbred WF Rats
Extramedullary Hematopoiesis
Somatotrophs
Myeloid Progenitor Cells
Rat control
cells
Tissue
Splenomegaly
splenomegaly
hematopoiesis
Regeneration

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Meier, W. A., Minshall, C. T., Arkins, S., French, R. A., Zachary, J. F., & Kelley, K. W. (1996). Growth hormone stimulates bone marrow and thymic reconstitution in aged rats. FASEB Journal, 10(6).

Growth hormone stimulates bone marrow and thymic reconstitution in aged rats. / Meier, William A.; Minshall, Christian T; Arkins, Sean; French, Richard A.; Zachary, James F.; Kelley, Keith W.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Meier, WA, Minshall, CT, Arkins, S, French, RA, Zachary, JF & Kelley, KW 1996, 'Growth hormone stimulates bone marrow and thymic reconstitution in aged rats', FASEB Journal, vol. 10, no. 6.
Meier WA, Minshall CT, Arkins S, French RA, Zachary JF, Kelley KW. Growth hormone stimulates bone marrow and thymic reconstitution in aged rats. FASEB Journal. 1996;10(6).
Meier, William A. ; Minshall, Christian T ; Arkins, Sean ; French, Richard A. ; Zachary, James F. ; Kelley, Keith W. / Growth hormone stimulates bone marrow and thymic reconstitution in aged rats. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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