Growth inhibition by protein kinase C late in mitogenesis

Chou Long Huang, Harlan E. Ives

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The importance of α-thrombin in the clotting cascade is well-known, but it is also a potent mitogen1-3. Like many other mitogens, thrombin causes receptor-mediated activation of a phosphatidy-linositol-specific phospholipase C (PLC), leading to the release of diacylglycerol and the subsequent activation of protein kinase C (refs 3-6). Protein kinase C is probably important in cell proliferation, as activation of this enzyme by phorbol esters promotes growth in many systems4-6. Some growth factors have tyrosine kinase activity and function without activation of PLC or protein kinase C7-9 . In this report we show that α-thrombin retains its mitogenicity in vascular smooth muscle cells depleted of protein kinase C. Phorbol-12-myristate-13-acetate (PMA) is found to be a potent growth inhibitor when added to vascular smooth muscle cells with α-thrombin. Moreover, growth inhibition is maximal when protein kinase C is activated 4 hours after exposure to thrombin, long after the completion of 'early events' induced by thrombin. Thus, PMA probes an event late in the G1 phase of the cell cyle or at the G1-S transition.

Original languageEnglish (US)
Pages (from-to)849-850
Number of pages2
JournalNature
Volume329
Issue number6142
StatePublished - 1987

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Thrombin
Protein Kinase C
Growth
Type C Phospholipases
Vascular Smooth Muscle
Smooth Muscle Myocytes
Acetates
Thrombin Receptors
Growth Inhibitors
Enzyme Activation
Diglycerides
G1 Phase
Phorbol Esters
Mitogens
Protein-Tyrosine Kinases
Protein Kinases
Intercellular Signaling Peptides and Proteins
Cell Proliferation
phorbol-12-myristate

ASJC Scopus subject areas

  • General

Cite this

Growth inhibition by protein kinase C late in mitogenesis. / Huang, Chou Long; Ives, Harlan E.

In: Nature, Vol. 329, No. 6142, 1987, p. 849-850.

Research output: Contribution to journalArticle

Huang, CL & Ives, HE 1987, 'Growth inhibition by protein kinase C late in mitogenesis', Nature, vol. 329, no. 6142, pp. 849-850.
Huang, Chou Long ; Ives, Harlan E. / Growth inhibition by protein kinase C late in mitogenesis. In: Nature. 1987 ; Vol. 329, No. 6142. pp. 849-850.
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