Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium

M. Brower, D. N. Carney, H. K. Oie, A. F. Gazdar, J. D. Minna

Research output: Contribution to journalArticle

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Abstract

We tested the ability of serum-free media to support the in vitro growth of human non-small cell lung carcinoma. A medium containing insulin, transferrin, sodium selenite, hydrocortisone, epidermal growth factor, and bovine serum albumin (1 mg/ml) with serum precoating of culture dishes (modified LA medium) supported three previously established cell lines of non-small cell lung cancer and prevented fibroblast proliferation in fresh tumor specimens but did not support long term tumor cell growth from fresh specimens. We added triiodothyronine, sodium pyruvate, and additional glutamine, insulin, and epidermal growth factor to modified LA medium, precoated with fibronectin and collagen instead of serum, and deleted bovine serum albumin, defining a new medium called ACL-3. ACL-3 medium alone supported the short term growth of 10 of 12 cell lines and the soft agarose cloning of 9 of 12 cell lines tested, and ACL-3 supplemented by an optimal concentration of bovine serum albumin (5 mg/ml) supported the long term growth of 10 of 12 cell lines tested. Moreover, we have grown tumor cells for more than 6 months from 11 of 33 (33%) consecutive fresh clinical specimens of human lung adenocarcinoma in ACL-3 with bovine serum albumin. ACL-3 medium provides a defined environment for the study of growth factor requirements of human non-small cell lung cancer and enhances our ability to grow human lung cancer, particularly adenocarcinoma, in vitro.

Original languageEnglish (US)
Pages (from-to)798-806
Number of pages9
JournalCancer Research
Volume46
Issue number2
StatePublished - 1986

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Serum-Free Culture Media
Bovine Serum Albumin
Non-Small Cell Lung Carcinoma
Cell Line
Growth
Epidermal Growth Factor
Insulin
Sodium Selenite
Neoplasms
Triiodothyronine
Transferrin
Glutamine
Serum
Pyruvic Acid
Fibronectins
Sepharose
Hydrocortisone
Organism Cloning
Lung Neoplasms
Intercellular Signaling Peptides and Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. / Brower, M.; Carney, D. N.; Oie, H. K.; Gazdar, A. F.; Minna, J. D.

In: Cancer Research, Vol. 46, No. 2, 1986, p. 798-806.

Research output: Contribution to journalArticle

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