Growth retardation and tumour inhibition by BRCA1

Jeffrey T. Holt, Marilyn E. Thompson, Csilla Szabo, Cheryl Robiǹson-Benion, Carlos L. Arteaga, Mary Claire King, Roy A. Jensen

Research output: Contribution to journalArticle

370 Citations (Scopus)

Abstract

Inherited mutations in BRCA1 predispose to breast and ovarian cancer, but the role of BRCA1 in sporadic breast and ovarian cancer has previously been elusive. Here, we show that retroviral transfer of the wild-type BRCA1 gene inhibits growth in vitro of all breast and ovarian cancer cell lines tested, but not colon or lung cancer cells or fibroblasts. Mutant BRCA1 has no effect on growth of breast cancer cells; ovarian cancer cell growth is not affected by BRCA1 mutations in the 5' portion of the gene, but is inhibited by 3' BRCA1 mutations. Development of MCF-7 tumours in nude mice is inhibited when MCF-7 cells are transfected with wild-type, but not mutant, BRCA1. Most importantly, among mice with established MCF-7 tumours, peritoneal treatment with a retroviral vector expressing wild-type BRCA1 significantly inhibits tumour growth and increased survival.

Original languageEnglish (US)
Pages (from-to)298-302
Number of pages5
JournalNature Genetics
Volume12
Issue number3
DOIs
StatePublished - Mar 1 1996

Fingerprint

Ovarian Neoplasms
Breast Neoplasms
Growth
Mutation
Neoplasms
BRCA1 Gene
MCF-7 Cells
Nude Mice
Colonic Neoplasms
Lung Neoplasms
Fibroblasts
Cell Line
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Holt, J. T., Thompson, M. E., Szabo, C., Robiǹson-Benion, C., Arteaga, C. L., King, M. C., & Jensen, R. A. (1996). Growth retardation and tumour inhibition by BRCA1. Nature Genetics, 12(3), 298-302. https://doi.org/10.1038/ng0396-298

Growth retardation and tumour inhibition by BRCA1. / Holt, Jeffrey T.; Thompson, Marilyn E.; Szabo, Csilla; Robiǹson-Benion, Cheryl; Arteaga, Carlos L.; King, Mary Claire; Jensen, Roy A.

In: Nature Genetics, Vol. 12, No. 3, 01.03.1996, p. 298-302.

Research output: Contribution to journalArticle

Holt, JT, Thompson, ME, Szabo, C, Robiǹson-Benion, C, Arteaga, CL, King, MC & Jensen, RA 1996, 'Growth retardation and tumour inhibition by BRCA1', Nature Genetics, vol. 12, no. 3, pp. 298-302. https://doi.org/10.1038/ng0396-298
Holt JT, Thompson ME, Szabo C, Robiǹson-Benion C, Arteaga CL, King MC et al. Growth retardation and tumour inhibition by BRCA1. Nature Genetics. 1996 Mar 1;12(3):298-302. https://doi.org/10.1038/ng0396-298
Holt, Jeffrey T. ; Thompson, Marilyn E. ; Szabo, Csilla ; Robiǹson-Benion, Cheryl ; Arteaga, Carlos L. ; King, Mary Claire ; Jensen, Roy A. / Growth retardation and tumour inhibition by BRCA1. In: Nature Genetics. 1996 ; Vol. 12, No. 3. pp. 298-302.
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