Gtp is required to stabilize and display transamidation activity of transglutaminase 2

Ju Hong Jeon, Sung Yup Cho, Chai Wan Kim, Dong Myung Shin, Joon Chul Kweon, Kyung Ho Choi, Sang Chul Park, In Gyu Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Transglutaminase 2 (TGase 2) is a bifunctional enzyme that catalyzes calcium-dependent transamidation and GTP binding/ hydrolysis. The transamidation activity is proposed to be associated with several neurodegenerative disorders such as Alzheimer's and Hungtinton's disease. However, the regulation mechanism by which TGase 2 causes neurodegeneration is unknown. In this study, we show that two activities of TGase 2 have a differential stability; transamidation activity is less stable than GTP hydrolytic activity, and that GTP was required to stabilize and to display transamidation activity. Moreover, GTP binding-defective mutant of TGase 2 did not show any transamidation activity in transfection experiments. These results indicate that GTP binding is crucial for transamidation activity of TGase 2, suggesting that protein cross-linking by TGase 2 might be associated with G-protein coupled receptor signaling system. Thus, our data could contribute to understand the regulation of TGase 2 activity and TGase 2-associated pathogenesis.

Original languageEnglish (US)
Pages (from-to)818-822
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume294
Issue number4
DOIs
StatePublished - 2002

Fingerprint

Guanosine Triphosphate
transglutaminase 2
G-Protein-Coupled Receptors
Neurodegenerative Diseases
Transfection
Hydrolysis
Alzheimer Disease
Calcium
Enzymes
Proteins
Experiments

Keywords

  • Enzyme stability
  • GTP binding/hydrolysis
  • Transamidation
  • Transglutaminase 2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Gtp is required to stabilize and display transamidation activity of transglutaminase 2. / Jeon, Ju Hong; Cho, Sung Yup; Kim, Chai Wan; Shin, Dong Myung; Kweon, Joon Chul; Choi, Kyung Ho; Park, Sang Chul; Kim, In Gyu.

In: Biochemical and Biophysical Research Communications, Vol. 294, No. 4, 2002, p. 818-822.

Research output: Contribution to journalArticle

Jeon, Ju Hong ; Cho, Sung Yup ; Kim, Chai Wan ; Shin, Dong Myung ; Kweon, Joon Chul ; Choi, Kyung Ho ; Park, Sang Chul ; Kim, In Gyu. / Gtp is required to stabilize and display transamidation activity of transglutaminase 2. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 294, No. 4. pp. 818-822.
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AU - Cho, Sung Yup

AU - Kim, Chai Wan

AU - Shin, Dong Myung

AU - Kweon, Joon Chul

AU - Choi, Kyung Ho

AU - Park, Sang Chul

AU - Kim, In Gyu

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