GTP/ggS-Induced phosphorylation of myosin light chain kinase in smooth muscle

Da Chun Tang; Yasutaka Kubota; Kristine E. Kamm; James T. Stull

doi:10.1016/0014-5793(93)80351-T

GTP/ggS-Induced phosphorylation of myosin light chain kinase in smooth muscle

Da Chun Tang, Yasutaka Kubota, Kristine E. Kamm, James T. Stull

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Phosphorylation of myosin light chain kinase by a Ca²⁺-dependent protein kinase increases the concentration of Ca²⁺/calmodulin required for half-maximal activation. The Ca²⁺ concentrations required for myosin light chain kinase phosphorylation in permeable smooth muscle are similar to those required for myosin light chain phosphorylation. Both GTPγS and carbachol increase the Ca²⁺ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. It is proposed that a similar G-protein mediated mechanism regulates the Ca²⁺-dependent phosphorylation of these two contractile proteins in smooth muscle.

Original language	English (US)
Pages (from-to)	272-275
Number of pages	4
Journal	FEBS Letters
Volume	331
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(93)80351-T
State	Published - Oct 4 1993

Keywords

GTPγS
Myosin light chain
Myosin light chain kinase
Protein phosphatase
Smooth muscle contraction

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(93)80351-T

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title = "GTP/ggS-Induced phosphorylation of myosin light chain kinase in smooth muscle",

abstract = "Phosphorylation of myosin light chain kinase by a Ca2+-dependent protein kinase increases the concentration of Ca2+/calmodulin required for half-maximal activation. The Ca2+ concentrations required for myosin light chain kinase phosphorylation in permeable smooth muscle are similar to those required for myosin light chain phosphorylation. Both GTPγS and carbachol increase the Ca2+ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. It is proposed that a similar G-protein mediated mechanism regulates the Ca2+-dependent phosphorylation of these two contractile proteins in smooth muscle.",

keywords = "GTPγS, Myosin light chain, Myosin light chain kinase, Protein phosphatase, Smooth muscle contraction",

author = "Tang, {Da Chun} and Yasutaka Kubota and Kamm, {Kristine E.} and Stull, {James T.}",

year = "1993",

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T1 - GTP/ggS-Induced phosphorylation of myosin light chain kinase in smooth muscle

AU - Tang, Da Chun

AU - Kubota, Yasutaka

AU - Kamm, Kristine E.

AU - Stull, James T.

PY - 1993/10/4

Y1 - 1993/10/4

N2 - Phosphorylation of myosin light chain kinase by a Ca2+-dependent protein kinase increases the concentration of Ca2+/calmodulin required for half-maximal activation. The Ca2+ concentrations required for myosin light chain kinase phosphorylation in permeable smooth muscle are similar to those required for myosin light chain phosphorylation. Both GTPγS and carbachol increase the Ca2+ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. It is proposed that a similar G-protein mediated mechanism regulates the Ca2+-dependent phosphorylation of these two contractile proteins in smooth muscle.

AB - Phosphorylation of myosin light chain kinase by a Ca2+-dependent protein kinase increases the concentration of Ca2+/calmodulin required for half-maximal activation. The Ca2+ concentrations required for myosin light chain kinase phosphorylation in permeable smooth muscle are similar to those required for myosin light chain phosphorylation. Both GTPγS and carbachol increase the Ca2+ sensitivity of myosin light chain kinase phosphorylation as well as light chain phosphorylation. It is proposed that a similar G-protein mediated mechanism regulates the Ca2+-dependent phosphorylation of these two contractile proteins in smooth muscle.

KW - GTPγS

KW - Myosin light chain

KW - Myosin light chain kinase

KW - Protein phosphatase

KW - Smooth muscle contraction

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GTP/ggS-Induced phosphorylation of myosin light chain kinase in smooth muscle

Abstract

Keywords

ASJC Scopus subject areas

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