Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer

Burt Cagir, Alyssa Gelmann, Jason Park, Tracy Fava, Alexander Tankelevitch, Edwin W. Bittner, Eric J. Weaver, Juan P. Palazzo, David Weinberg, Robert D. Fry, Scott A. Waldman

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Abstract

Background: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors. Objective: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease. Design: Case-control study. Setting: Tertiary care academic medical center. Patients: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node- negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection. Measurements: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7). Conclusions: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging.

Original languageEnglish (US)
Pages (from-to)805-812
Number of pages8
JournalAnnals of Internal Medicine
Volume131
Issue number11
StatePublished - Dec 7 1999

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Colorectal Neoplasms
Biomarkers
Messenger RNA
Lymph Nodes
Reverse Transcriptase Polymerase Chain Reaction
Carcinoembryonic Antigen
enterotoxin receptor
Paraffin
Intestinal Neoplasms
Recurrence
Neoplasm Micrometastasis
Neoplasm Staging
Guanylate Cyclase
Tertiary Healthcare
Case-Control Studies
Odds Ratio
RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cagir, B., Gelmann, A., Park, J., Fava, T., Tankelevitch, A., Bittner, E. W., ... Waldman, S. A. (1999). Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. Annals of Internal Medicine, 131(11), 805-812.

Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. / Cagir, Burt; Gelmann, Alyssa; Park, Jason; Fava, Tracy; Tankelevitch, Alexander; Bittner, Edwin W.; Weaver, Eric J.; Palazzo, Juan P.; Weinberg, David; Fry, Robert D.; Waldman, Scott A.

In: Annals of Internal Medicine, Vol. 131, No. 11, 07.12.1999, p. 805-812.

Research output: Contribution to journalArticle

Cagir, B, Gelmann, A, Park, J, Fava, T, Tankelevitch, A, Bittner, EW, Weaver, EJ, Palazzo, JP, Weinberg, D, Fry, RD & Waldman, SA 1999, 'Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer', Annals of Internal Medicine, vol. 131, no. 11, pp. 805-812.
Cagir B, Gelmann A, Park J, Fava T, Tankelevitch A, Bittner EW et al. Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. Annals of Internal Medicine. 1999 Dec 7;131(11):805-812.
Cagir, Burt ; Gelmann, Alyssa ; Park, Jason ; Fava, Tracy ; Tankelevitch, Alexander ; Bittner, Edwin W. ; Weaver, Eric J. ; Palazzo, Juan P. ; Weinberg, David ; Fry, Robert D. ; Waldman, Scott A. / Guanylyl cyclase C messenger RNA is a biomarker for recurrent stage II colorectal cancer. In: Annals of Internal Medicine. 1999 ; Vol. 131, No. 11. pp. 805-812.
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abstract = "Background: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors. Objective: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease. Design: Case-control study. Setting: Tertiary care academic medical center. Patients: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node- negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection. Measurements: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95{\%} CI, 1.1 to 756.7). Conclusions: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging.",
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AU - Park, Jason

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AU - Tankelevitch, Alexander

AU - Bittner, Edwin W.

AU - Weaver, Eric J.

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AU - Weinberg, David

AU - Fry, Robert D.

AU - Waldman, Scott A.

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N2 - Background: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors. Objective: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease. Design: Case-control study. Setting: Tertiary care academic medical center. Patients: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node- negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection. Measurements: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7). Conclusions: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging.

AB - Background: Patients with stage II colorectal cancer and no histologic evidence of lymph node invasion develop recurrent disease, presumably because of undetected micrometastases. Guanylyl cyclase C is expressed by intestinal and colorectal cancer cells but not by extraintestinal tissues or tumors. Objective: To examine the expression of guanylyl cyclase C messenger RNA (mRNA) in lymph nodes of patients with node-negative colorectal cancer who did and did not have recurrent disease. Design: Case-control study. Setting: Tertiary care academic medical center. Patients: Paraffin-embedded lymph nodes were obtained from 21 patients with histologically confirmed node- negative colorectal cancer who had undergone resection. Controls included 11 patients without disease recurrence 6 or more years after resection, and case-patients included 10 patients whose disease recurred up to 3 years after resection. Measurements: Sections of paraffin-embedded lymph nodes were obtained from each patient and were pooled, and their RNA was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: Guanylyl cyclase C mRNA was expressed in lymph nodes from all patients with recurrent disease but not in those from patients without recurrent disease (P = 0.004). Nested RT-PCR that used primers for carcinoembryonic antigen, a marker for colorectal cancer, identified carcinoembryonic antigen mRNA in lymph nodes from only 1 of 10 patients with recurrent disease and those from 0 of 11 patients without recurrent disease. The odds ratio for death associated with expression of guanylyl cyclase C mRNA in regional lymph nodes was 15.0 (95% CI, 1.1 to 756.7). Conclusions: Expression of guanylyl cyclase C mRNA in lymph nodes is associated with recurrence of colorectal cancer in patients with stage II disease. Analysis of guanylyl cyclase mRNA expression by RT-PCR may be useful for colorectal cancer staging.

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