H-Scan Format for Classification of Ultrasound Scatterers and Matched Comparison to Histology Measurements

Mawia Khairalseed, Girdhari Rijal, Kenneth Hoyt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

H-scan imaging is a new ultrasound (US) technique used to visualize the relative size of acoustic scatterers. The purpose of this study was to evaluate the sensitivity of H-scan US imaging to scatterer size and comparison to histological sections of tumor tissue. Image data was acquired using a programmable US scanner (Vantage 256, Verasonics Inc) equipped with a 256-element L22-8v capacitive micromachined ultrasonic transducer (CMUT, Kolo Medical). To generate the H-scan US image, three parallel convolution filters were applied to the radiofrequency (RF) data sequences to measure the relative strength of the backscattered US signals. H-scan US imaging was used to image a gelatin-based heterogenous phantom and breast tumor-bearing mice (N = 4). Excised tumor tissue underwent histologic processing and the cells were segmented to compute physical size measurements at the cellular level followed by spatial correlation with H-scan US image features. The in vitro results show that there was an improvement in the contrast-to-noise ratio (CNR) of 44.1% for H-scan compared to B-scan US imaging. Preliminary animal studies revealed there was a statistically significant relationship between H-scan US and physical size measures at the cell level (R2 > 0.95, p < 0.02). Overall, this study details the first experimental evidence that H-scan US image findings are directly related to physical cell size of the underlying bulk tumor tissue.

Original languageEnglish (US)
Title of host publicationISBI 2020 - 2020 IEEE International Symposium on Biomedical Imaging
PublisherIEEE Computer Society
Pages1820-1823
Number of pages4
ISBN (Electronic)9781538693308
DOIs
StatePublished - Apr 2020
Event17th IEEE International Symposium on Biomedical Imaging, ISBI 2020 - Iowa City, United States
Duration: Apr 3 2020Apr 7 2020

Publication series

NameProceedings - International Symposium on Biomedical Imaging
Volume2020-April
ISSN (Print)1945-7928
ISSN (Electronic)1945-8452

Conference

Conference17th IEEE International Symposium on Biomedical Imaging, ISBI 2020
CountryUnited States
CityIowa City
Period4/3/204/7/20

Keywords

  • CMUT
  • H-scan ultrasound
  • spatial angular compounding
  • tissue characterization
  • ultrasound imaging

ASJC Scopus subject areas

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Khairalseed, M., Rijal, G., & Hoyt, K. (2020). H-Scan Format for Classification of Ultrasound Scatterers and Matched Comparison to Histology Measurements. In ISBI 2020 - 2020 IEEE International Symposium on Biomedical Imaging (pp. 1820-1823). [9098439] (Proceedings - International Symposium on Biomedical Imaging; Vol. 2020-April). IEEE Computer Society. https://doi.org/10.1109/ISBI45749.2020.9098439