H2-M3 presents a nonformylated viral epitope to CTLs generated in vitro

Derek E. Byers, Kirsten Fischer Lindahl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Most CTL responses to epitopes from influenza virus are restricted by MHC class Ia molecules. However, a synthetic peptide corresponding to residues 173 to 190 of influenza A/JAP/305/57 hemagglutinin (HA) can induce, in vitro, a CTI, response to peptide presented by a mouse class Ib molecule encoded by a gene telomeric to H2-Q. Here, we identify the molecule as H2-M3 and show that the last five residues of HA173-190, MLIIW, is the minimal epitope for CTL recognition. Cells that express M3(wt), from C57BL/6 or BALB/c mice, are sensitized by both MLIIW and the longer peptide HA173-190, whereas cells that express M3(r), from A.CA or B10.M mice, are sensitized only by MLIIW; a single amino acid change at residue 31 (V→M) of M3 accounts for this difference. Although M3-restricted CTLs preferably recognize N- formylated epitopes, i.e., those of mitochondrial or prokaryotic origin, our findings show that M3-restricted primary CTL responses can be generated in vitro against nonformylated epitopes.

Original languageEnglish (US)
Pages (from-to)90-96
Number of pages7
JournalJournal of Immunology
Volume161
Issue number1
StatePublished - 1998

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Epitopes
Peptides
Hemagglutinins
Orthomyxoviridae
Human Influenza
Amino Acids
In Vitro Techniques
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

H2-M3 presents a nonformylated viral epitope to CTLs generated in vitro. / Byers, Derek E.; Lindahl, Kirsten Fischer.

In: Journal of Immunology, Vol. 161, No. 1, 1998, p. 90-96.

Research output: Contribution to journalArticle

Byers, DE & Lindahl, KF 1998, 'H2-M3 presents a nonformylated viral epitope to CTLs generated in vitro', Journal of Immunology, vol. 161, no. 1, pp. 90-96.
Byers, Derek E. ; Lindahl, Kirsten Fischer. / H2-M3 presents a nonformylated viral epitope to CTLs generated in vitro. In: Journal of Immunology. 1998 ; Vol. 161, No. 1. pp. 90-96.
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