H2-M3wt-restricted, Listeria monocytogenes-specific CD8 T cells recognize a novel, hydrophobic, protease-resistant, periodate-sensitive antigen

Chandrasekaran Nataraj, Melinda L. Brown, Rebecca M. Poston, Said M. Shawar, Robert R. Rich, Kirsten Fischer Lindahl, Roger J. Kurlander

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Mice infected with Listeria monocytogenes (LM) generate H2-M3wt-restricted CD8 effectors which recognize a heat-killed LM-associated antigen (HAA) presented by macrophages. To characterize HAA, we extracted a bioactive component from LM using SDS or NaOH. Extracted HAA aggregated in hydrophilic solvents but dissociated in the presence of SDS into a smaller subunit which migrated in Sephadex G-200 between chymotrypsinogen (25 kDa) and cytochrome c (12.5 kDa). HAA bioactivity and size was unaffected by proteinase K under conditions which degraded virtually all detectable protein. HAA was also unaffected by other proteases, RNase and DNase, but HAA bioactivity was destroyed by periodate, an agent that degrades carbohydrates. These studies demonstrate that H2-M3wt can present a hydrophobic, non-peptide, microbial antigen, probably glycolipid in origin, to CD8 T cells.

Original languageEnglish (US)
Pages (from-to)367-378
Number of pages12
JournalInternational Immunology
Volume8
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • Antigen presentation
  • Antigen processing
  • Antigen-presenting cells
  • MHC class I products
  • Macrophage
  • T cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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