Haemophilus influenzae outer membrane vesicle-induced blood-brain barrier permeability during experimental meningitis

N. Wispelwey, E. J. Hansen, W. M. Scheld

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Haemophilus influenzae type b (Hib) lipopolysaccharide (LPS) may be present in the cerebrospinal fluid largely as part of outer membrane vesicles (OMV), which could possibly alter its activity. Similar to inoculation of purified Hib LPS, intracisternal inoculation of Hib OMV into adult rats resulted in dose- and time-dependent increases in blood-brain barrier permeability. Polymyxin B, but not an oligosaccharide-specific monoclonal antibody, significantly inhibited the activity of Hib OMV. No change in blood-brain barrier permeability occurred in leukopenic rats inoculated with Hib OMV. Hib OMV was as active as purified Hib LPS on a weight basis and therefore appears to be a relevant vehicle for the delivery of LPS during meningitis.

Original languageEnglish (US)
Pages (from-to)2559-2562
Number of pages4
JournalInfection and Immunity
Volume57
Issue number8
StatePublished - 1989

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Haemophilus influenzae type b
Haemophilus influenzae
Blood-Brain Barrier
Meningitis
Permeability
Membranes
Lipopolysaccharides
Polymyxin B
Oligosaccharides
Cerebrospinal Fluid
Monoclonal Antibodies
Weights and Measures

ASJC Scopus subject areas

  • Immunology

Cite this

Haemophilus influenzae outer membrane vesicle-induced blood-brain barrier permeability during experimental meningitis. / Wispelwey, N.; Hansen, E. J.; Scheld, W. M.

In: Infection and Immunity, Vol. 57, No. 8, 1989, p. 2559-2562.

Research output: Contribution to journalArticle

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