HDAC7 inhibits osteoclastogenesis by reversing RANKL-triggered β-catenin switch

Zixue Jin, Wei Wei, Paul C. Dechow, Yihong Wan

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The bone-resorbing osteoclast is essential for skeletal remodeling, yet its deregulation contributes to diseases such as osteoporosis and cancer bone metastasis. Here we identify histone deacetylase 7 (HDAC7) as a key negative regulator of osteoclastogenesis and bone resorption using both in vitro cellular and molecular analyses and in vivo characterization of conditional HDAC7-knockout mice. Bone marrow osteoclast differentiation assays reveal that HDAC7 overexpression suppresses, whereas HDAC7 deletion enhances, osteoclastogenesis. Mechanistically, in the absence of receptor activator of nuclear factor κ-B ligand (RANKL), HDAC7 attenuates β-catenin function and cyclin D1 expression, thereby reducing precursor proliferation; upon RANKL activation, HDAC7 suppresses NFATc1 and prevents β-catenin down-regulation, thereby blocking osteoclast differentiation. Consequently, HDAC7 deletion in the osteoclast lineage results in a 26% reduction in bone mass (P = 0.003) owing to 102% elevated bone resorption (P = 0.01). These findings are clinically significant in light of the remarkable therapeutic potentials of HDAC inhibitors for several diseases such as cancer, diabetes, and neurodegeneration.

Original languageEnglish (US)
Pages (from-to)325-335
Number of pages11
JournalMolecular Endocrinology
Volume27
Issue number2
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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