Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1

A. B. Firulli, D. G. McFadden, Q. Lin, D. Srivastava, E. N. Olson

Research output: Contribution to journalArticle

266 Citations (Scopus)

Abstract

The basic helix-loop-helix (bHLH) transcription factors, Hand1 and Hand2 (refs 1,2), also called eHand/Hxt/Thing1 and dHand/Hed/Thing2 (refs 3,4), respectively, are expressed in the heart and certain neural-crest derivatives during embryogenesis. In addition, Hand1 is expressed in extraembryonic membranes, whereas Hand2 is expressed in the deciduum. Previous studies have demonstrated that Hand2 is required for formation of the right ventricle of the heart and the aortic arch arteries. We have generated a germline mutation in the mouse Hand1 gene by replacing the first coding exon with a β- galactosidase reporter gene. Embryos homozygous for the Hand1 null allele died between embryonic days 8.5 and 9.5 and exhibited yolk sac abnormalities due to a deficiency in extraembryonic mesoderm. Heart development was also perturbed and did not progress beyond the cardiac-looping stage. Our results demonstrate important roles for Hand1 in extraembryonic mesodermal and heart development.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalNature Genetics
Volume18
Issue number3
DOIs
StatePublished - 1998

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Basic Helix-Loop-Helix Transcription Factors
Embryonic Structures
Galactosidases
Extraembryonic Membranes
Decidua
Yolk Sac
Germ-Line Mutation
Neural Crest
Mesoderm
Thoracic Aorta
Reporter Genes
Heart Ventricles
Embryonic Development
Exons
Arteries
Alleles
Genes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1. / Firulli, A. B.; McFadden, D. G.; Lin, Q.; Srivastava, D.; Olson, E. N.

In: Nature Genetics, Vol. 18, No. 3, 1998, p. 266-270.

Research output: Contribution to journalArticle

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