HEB, a Helix-Loop-Helix Protein Related to E2A and ITF2 That Can Modulate the DNA-Binding Ability of Myogenic Regulatory Factors

Jing Shan Hu, Eric N. Olson, Robert E. Kingston

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

Proteins containing the basic-helix-loop-helix (B-HLH) domain have been shown to be important in regulating cellular differentiation. We have isolated a cDNA for a human B-HLH factor, denoted HEB, that shares nearly complete identity in the B-HLH domain with the immunoglobulin enhancer binding proteins encoded by the E2A and ITF2 genes (E proteins). Functional characterization of the protein expressed from this cDNA indicates that HEB is a third member of the E-protein class of B-HLH factors. HEB mRNA was found to be expressed in several tissues and cell types, including skeletal muscle, thymus, and a B-cell line. HEB, ITF2, and the E12 product of the E2A gene all bound to a similar spectrum of E-box sequences as homo-oligomers. All three factors also formed hetero-oligomers with myogenin, and the DNA-binding specificity and binding off-rates (dissociation rates) were modulated after hetero-oligomerization. Both homo-and hetero-oligomers of these proteins were able to distinguish between very closely related E-box sequences. In addition, HEB was shown to form hetero-oligomers with the E12 and ITF2 proteins. Finally, HEB was able to activate gene expression. These data demonstrate that HEB shares characteristics with other E proteins and show that HEB can interact with members of both the myogenic regulatory class and the E-protein class of B-HLH factors. HEB is therefore likely to play an important role in regulating lineage-specific gene expression.

Original languageEnglish (US)
Pages (from-to)1031-1042
Number of pages12
JournalMolecular and Cellular Biology
Volume12
Issue number3
StatePublished - Mar 1992

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Myogenic Regulatory Factors
DNA
Helix-Loop-Helix Motifs
Proteins
E-Box Elements
Complementary DNA
Myogenin
Gene Expression
NF-kappa B
Thymus Gland
Skeletal Muscle
B-Lymphocytes
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

HEB, a Helix-Loop-Helix Protein Related to E2A and ITF2 That Can Modulate the DNA-Binding Ability of Myogenic Regulatory Factors. / Hu, Jing Shan; Olson, Eric N.; Kingston, Robert E.

In: Molecular and Cellular Biology, Vol. 12, No. 3, 03.1992, p. 1031-1042.

Research output: Contribution to journalArticle

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abstract = "Proteins containing the basic-helix-loop-helix (B-HLH) domain have been shown to be important in regulating cellular differentiation. We have isolated a cDNA for a human B-HLH factor, denoted HEB, that shares nearly complete identity in the B-HLH domain with the immunoglobulin enhancer binding proteins encoded by the E2A and ITF2 genes (E proteins). Functional characterization of the protein expressed from this cDNA indicates that HEB is a third member of the E-protein class of B-HLH factors. HEB mRNA was found to be expressed in several tissues and cell types, including skeletal muscle, thymus, and a B-cell line. HEB, ITF2, and the E12 product of the E2A gene all bound to a similar spectrum of E-box sequences as homo-oligomers. All three factors also formed hetero-oligomers with myogenin, and the DNA-binding specificity and binding off-rates (dissociation rates) were modulated after hetero-oligomerization. Both homo-and hetero-oligomers of these proteins were able to distinguish between very closely related E-box sequences. In addition, HEB was shown to form hetero-oligomers with the E12 and ITF2 proteins. Finally, HEB was able to activate gene expression. These data demonstrate that HEB shares characteristics with other E proteins and show that HEB can interact with members of both the myogenic regulatory class and the E-protein class of B-HLH factors. HEB is therefore likely to play an important role in regulating lineage-specific gene expression.",
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