Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of cubitus interruptus

Wensheng Zhang, Yun Zhao, Chao Tong, Gelin Wang, Bing Wang, Jianhang Jia, Jin Jiang

Research output: Contribution to journalArticle

138 Citations (Scopus)

Abstract

Hedgehog (Hh) proteins control animal development by regulating the Gli/Ci family of transcription factors. In Drosophila, Hh counteracts phosphorylation by PKA, GSK3, and CKI to prevent Cubitus interruptus (Ci) processing through unknown mechanisms. Here, we show that these kinases physically interact with the kinesin-like protein Costal2 (Cos2) to control Ci processing and that Hh inhibits such interaction. Cos2 is required for Ci phosphorylation in vivo, and Cos2-immunocomplexes (Cos2IPs) phosphorylate Ci and contain PKA, GSK3, and CKI. By using a Kinesin-Cos2 chimeric protein that carries Cos2-interacting proteins to the microtubule plus end, we demonstrated that these kinases bind Cos2 in intact cells. PKA, GSK3, and CKI directly bind the N- and C-terminal regions of Cos2, both of which are essential for Ci processing. Finally, we showed that Hh signaling inhibits Cos2-kinase complex formation. We propose that Cos2 recruits multiple kinases to efficiently phosphorylate Ci and that Hh inhibits Ci phosphorylation by specifically interfering with kinase recruitment.

Original languageEnglish (US)
Pages (from-to)267-278
Number of pages12
JournalDevelopmental Cell
Volume8
Issue number2
DOIs
StatePublished - Feb 2005

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Phosphorylation
Hedgehogs
Phosphotransferases
Processing
Kinesin
Hedgehog Proteins
Microtubule Proteins
Proteins
Drosophila
Animals
Transcription Factors

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of cubitus interruptus. / Zhang, Wensheng; Zhao, Yun; Tong, Chao; Wang, Gelin; Wang, Bing; Jia, Jianhang; Jiang, Jin.

In: Developmental Cell, Vol. 8, No. 2, 02.2005, p. 267-278.

Research output: Contribution to journalArticle

Zhang, Wensheng ; Zhao, Yun ; Tong, Chao ; Wang, Gelin ; Wang, Bing ; Jia, Jianhang ; Jiang, Jin. / Hedgehog-regulated Costal2-kinase complexes control phosphorylation and proteolytic processing of cubitus interruptus. In: Developmental Cell. 2005 ; Vol. 8, No. 2. pp. 267-278.
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