Hedgehog signaling and the retina

Insights into the mechanisms controlling the proliferative properties of neural precursors

Morgane Locker, Michalis Agathocleous, Marcos A. Amato, Karine Parain, William A. Harris, Muriel Perron

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit. Here we demonstrate a novel role for Hedgehog signaling in speeding up the cell cycle in the developing retina by reducing the length of G1 and G2 phases. These fast cycling cells tend to exit the cell cycle early. Conversely, retinal progenitors with blocked Hedgehog signaling cycle more slowly, with longer G1 and G2 phases, and remain in the cell cycle longer. Hedgehog may modulate cell cycle kinetics through activation of the key cell cycle activators cyclin D1, cyclin A2, cyclin B1, and cdc25C. These findings support a role for Hedgehog in regulating the conversion from slow cycling stem cells to fast cycling transient amplifying progenitors that are closer to cell cycle exit.

Original languageEnglish (US)
Pages (from-to)3036-3048
Number of pages13
JournalGenes and Development
Volume20
Issue number21
DOIs
StatePublished - Nov 1 2006

Fingerprint

Hedgehogs
Retina
Cell Cycle
G2 Phase
G1 Phase
Cyclin A2
Cyclin B1
Cyclin D1
Vertebrates
Stem Cells
Cell Proliferation

Keywords

  • Cdc25C
  • Cell cycle exit
  • Cell cycle kinetics
  • Cyclin
  • Hedgehog
  • Retinal stem cells
  • Xenopus
  • Zebrafish

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Hedgehog signaling and the retina : Insights into the mechanisms controlling the proliferative properties of neural precursors. / Locker, Morgane; Agathocleous, Michalis; Amato, Marcos A.; Parain, Karine; Harris, William A.; Perron, Muriel.

In: Genes and Development, Vol. 20, No. 21, 01.11.2006, p. 3036-3048.

Research output: Contribution to journalArticle

Locker, Morgane ; Agathocleous, Michalis ; Amato, Marcos A. ; Parain, Karine ; Harris, William A. ; Perron, Muriel. / Hedgehog signaling and the retina : Insights into the mechanisms controlling the proliferative properties of neural precursors. In: Genes and Development. 2006 ; Vol. 20, No. 21. pp. 3036-3048.
@article{0a1731aa8b384ff9825fdbd290eb8f73,
title = "Hedgehog signaling and the retina: Insights into the mechanisms controlling the proliferative properties of neural precursors",
abstract = "Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit. Here we demonstrate a novel role for Hedgehog signaling in speeding up the cell cycle in the developing retina by reducing the length of G1 and G2 phases. These fast cycling cells tend to exit the cell cycle early. Conversely, retinal progenitors with blocked Hedgehog signaling cycle more slowly, with longer G1 and G2 phases, and remain in the cell cycle longer. Hedgehog may modulate cell cycle kinetics through activation of the key cell cycle activators cyclin D1, cyclin A2, cyclin B1, and cdc25C. These findings support a role for Hedgehog in regulating the conversion from slow cycling stem cells to fast cycling transient amplifying progenitors that are closer to cell cycle exit.",
keywords = "Cdc25C, Cell cycle exit, Cell cycle kinetics, Cyclin, Hedgehog, Retinal stem cells, Xenopus, Zebrafish",
author = "Morgane Locker and Michalis Agathocleous and Amato, {Marcos A.} and Karine Parain and Harris, {William A.} and Muriel Perron",
year = "2006",
month = "11",
day = "1",
doi = "10.1101/gad.391106",
language = "English (US)",
volume = "20",
pages = "3036--3048",
journal = "Genes and Development",
issn = "0890-9369",
publisher = "Cold Spring Harbor Laboratory Press",
number = "21",

}

TY - JOUR

T1 - Hedgehog signaling and the retina

T2 - Insights into the mechanisms controlling the proliferative properties of neural precursors

AU - Locker, Morgane

AU - Agathocleous, Michalis

AU - Amato, Marcos A.

AU - Parain, Karine

AU - Harris, William A.

AU - Perron, Muriel

PY - 2006/11/1

Y1 - 2006/11/1

N2 - Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit. Here we demonstrate a novel role for Hedgehog signaling in speeding up the cell cycle in the developing retina by reducing the length of G1 and G2 phases. These fast cycling cells tend to exit the cell cycle early. Conversely, retinal progenitors with blocked Hedgehog signaling cycle more slowly, with longer G1 and G2 phases, and remain in the cell cycle longer. Hedgehog may modulate cell cycle kinetics through activation of the key cell cycle activators cyclin D1, cyclin A2, cyclin B1, and cdc25C. These findings support a role for Hedgehog in regulating the conversion from slow cycling stem cells to fast cycling transient amplifying progenitors that are closer to cell cycle exit.

AB - Hedgehog signaling has been linked to cell proliferation in a variety of systems; however, its effects on the cell cycle have not been closely studied. In the vertebrate retina, Hedgehog's effects are controversial, with some reports emphasizing increased proliferation and others pointing to a role in cell cycle exit. Here we demonstrate a novel role for Hedgehog signaling in speeding up the cell cycle in the developing retina by reducing the length of G1 and G2 phases. These fast cycling cells tend to exit the cell cycle early. Conversely, retinal progenitors with blocked Hedgehog signaling cycle more slowly, with longer G1 and G2 phases, and remain in the cell cycle longer. Hedgehog may modulate cell cycle kinetics through activation of the key cell cycle activators cyclin D1, cyclin A2, cyclin B1, and cdc25C. These findings support a role for Hedgehog in regulating the conversion from slow cycling stem cells to fast cycling transient amplifying progenitors that are closer to cell cycle exit.

KW - Cdc25C

KW - Cell cycle exit

KW - Cell cycle kinetics

KW - Cyclin

KW - Hedgehog

KW - Retinal stem cells

KW - Xenopus

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=33751085162&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751085162&partnerID=8YFLogxK

U2 - 10.1101/gad.391106

DO - 10.1101/gad.391106

M3 - Article

VL - 20

SP - 3036

EP - 3048

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 21

ER -