HeLaTG cells have mitochondrial DNA inserted into the c-myc oncogene

Jerry W. Shay, Tsuneyoshi Baba, Qimin Zhan, Naohisa Kamimura, Jennifer A. Cuthbert

Research output: Contribution to journalArticle

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Abstract

To determine whether mitochondrial DNA (mtDNA) fragments found within the nucleus are transcribed, we have differentially screened a HeLaTG cDNA library. A clone that hybridized to mtDNA as well as to c-myc was identified. Analysis of the cDNA disclosed that it contained a mtDNA sequence, encoding cytochrome-c oxidase subunit III (coxIII) that was contiguous with and 5′ of a c-myc sequence corresponding to part of exon 2 and exon 3. Hybridization of Seal-digested DNA with a 1.05kb c-myc probe revealed a unique band in HeLaTG cells, as well as a band common to HeLaTG and 13 other cell types examined. Solution hybridization of HeLaTG RNA with a radiolabeled, single-stranded cDNA probe containing the coxIII-c-wiyc junction demonstrated a nuclease-resistant band that matched the full length of the junctional cDNA probe. A smaller band that equaled the size of the c-myc portion alone was also detected. Only the smaller band coinciding with the c-myc sequences was protected from nuclease digestion by RNA from other cells. When a radiolabeled probe synthesized in the opposite orientation was used, nuclease-resistant bands equal in length to the coxIII portion of the probe were detected after hybridization with RNA from all cells. These results indicate that insertion of mtDNA fragments into nuclear genes occurs and that subsequent transcription of a 'chimaeric' or 'fusion' mRNA containing both mitochondrial and nuclear sequences can ensue.

Original languageEnglish (US)
Pages (from-to)1869-1874
Number of pages6
JournalOncogene
Volume6
Issue number10
StatePublished - Oct 1991

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myc Genes
Mitochondrial DNA
Complementary DNA
Exons
Oxidoreductases
RNA
Electron Transport Complex IV
Ribonucleases
Gene Library
Digestion
Clone Cells
Messenger RNA
DNA
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

HeLaTG cells have mitochondrial DNA inserted into the c-myc oncogene. / Shay, Jerry W.; Baba, Tsuneyoshi; Zhan, Qimin; Kamimura, Naohisa; Cuthbert, Jennifer A.

In: Oncogene, Vol. 6, No. 10, 10.1991, p. 1869-1874.

Research output: Contribution to journalArticle

Shay, JW, Baba, T, Zhan, Q, Kamimura, N & Cuthbert, JA 1991, 'HeLaTG cells have mitochondrial DNA inserted into the c-myc oncogene', Oncogene, vol. 6, no. 10, pp. 1869-1874.
Shay, Jerry W. ; Baba, Tsuneyoshi ; Zhan, Qimin ; Kamimura, Naohisa ; Cuthbert, Jennifer A. / HeLaTG cells have mitochondrial DNA inserted into the c-myc oncogene. In: Oncogene. 1991 ; Vol. 6, No. 10. pp. 1869-1874.
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