Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils

Tatsuo Ikeno, Hiroyoshi Ota, Atsushi Sugiyama, Kimitaka Ishida, Tsutomu Katsuyama, Robert M. Genta, Seiji Kawasaki

Research output: Contribution to journalArticle

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Abstract

The establishment of persisting Helicobacter pylori infection in laboratory animals has been difficult, but in 1996 Hirayama reported the development of a successful Mongolian gerbil model. The present study was undertaken with two aims: to better characterize the normal histological structure and histochemical properties of the gastric mucosa of the Mongolian gerbil; and to evaluate the progression of the histopathological features of H. pylori-induced gastritis in this animal model for one year after the experimental infection. Seventy-five Mongolian gerbils were used. Mongolian gerbils were sacrificed at 2, 4, 8, 12, 26, 38, and 52 weeks after H. pylori inoculation. Sections prepared from stomachs immediately fixed in Carnoy's solution were stained with hematoxylin and eosin and Alcian blue at pH 2.5/periodic acid-Schiff, a dual staining consisting of the galactose oxidase-cold thionin Schiff reaction and paradoxical Concanavalin A staining, and with immunostaining for H. pylori and BrdU. H. pylori infection induced in the Mongolian gerbil a chronic active gastritis, in which a marked mucosal infiltration of neutrophils on a background of chronic inflammation became detectable 4 weeks after inoculation and continued up to 52 weeks. Intestinal metaplasia and gastric ulcers appeared after 26 weeks in some of the animals, whereas others developed multiple hyperplastic polyps. The Mongolian gerbil represents a novel and useful model for the study of H. pylori-induced chronic active gastritis and may lend itself to the investigation of the epithelial alterations that lead to intestinal metaplasia and gastric neoplasia.

Original languageEnglish (US)
Pages (from-to)951-960
Number of pages10
JournalAmerican Journal of Pathology
Volume154
Issue number3
StatePublished - Mar 1999

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Gerbillinae
Metaplasia
Gastritis
Stomach Ulcer
Helicobacter pylori
Helicobacter Infections
Stomach
Thionins
Galactose Oxidase
Staining and Labeling
Alcian Blue
Periodic Acid
Neutrophil Infiltration
Laboratory Animals
Bromodeoxyuridine
Hematoxylin
Eosine Yellowish-(YS)
Concanavalin A
Polyps
Gastric Mucosa

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Ikeno, T., Ota, H., Sugiyama, A., Ishida, K., Katsuyama, T., Genta, R. M., & Kawasaki, S. (1999). Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils. American Journal of Pathology, 154(3), 951-960.

Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils. / Ikeno, Tatsuo; Ota, Hiroyoshi; Sugiyama, Atsushi; Ishida, Kimitaka; Katsuyama, Tsutomu; Genta, Robert M.; Kawasaki, Seiji.

In: American Journal of Pathology, Vol. 154, No. 3, 03.1999, p. 951-960.

Research output: Contribution to journalArticle

Ikeno, T, Ota, H, Sugiyama, A, Ishida, K, Katsuyama, T, Genta, RM & Kawasaki, S 1999, 'Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils', American Journal of Pathology, vol. 154, no. 3, pp. 951-960.
Ikeno, Tatsuo ; Ota, Hiroyoshi ; Sugiyama, Atsushi ; Ishida, Kimitaka ; Katsuyama, Tsutomu ; Genta, Robert M. ; Kawasaki, Seiji. / Helicobacter pylori-induced chronic active gastritis, intestinal metaplasia, and gastric ulcer in Mongolian gerbils. In: American Journal of Pathology. 1999 ; Vol. 154, No. 3. pp. 951-960.
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