Hematopoietic Stem Cells Do Not Depend on N-Cadherin to Regulate Their Maintenance

Mark J. Kiel, Melih Acar, Glenn L. Radice, Sean J. Morrison

Research output: Contribution to journalArticle

133 Scopus citations

Abstract

According to the "osteoblastic niche" model, hematopoietic stem cells (HSCs) are maintained by N-cadherin-mediated homophilic adhesion to osteoblasts at the bone marrow endosteum. In contrast to this model, we cannot detect N-cadherin expression by HSCs, and most HSCs do not localize to the endosteal surface. It has nonetheless been suggested that HSCs express low levels of N-cadherin that regulate HSC maintenance. To test this, we conditionally deleted N-cadherin from HSCs and other hematopoietic cells in adult Mx-1-Cre+N-cadherinfl/- mice. N-cadherin deficiency had no detectable effect on HSC maintenance or hematopoiesis. N-cadherin deficiency did not affect bone marrow cellularity or lineage composition, the numbers of colony-forming progenitors, the frequency of HSCs, the ability of HSCs to sustain hematopoiesis over time, or their ability to reconstitute irradiated mice in primary or secondary transplants. Loss of N-cadherin does not lead to HSC depletion. N-cadherin expression by HSCs is not necessary for niche function.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalCell Stem Cell
Volume4
Issue number2
DOIs
StatePublished - Feb 6 2009

Keywords

  • STEMCELL

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Cell Biology

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