Hemerythrins in the microaerophilic bacterium Campylobacter jejuni help protect key iron-sulphur cluster enzymes from oxidative damage

John J. Kendall, Angelica M. Barrero-Tobon, David R. Hendrixson, David J. Kelly

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Summary: Microaerophilic bacteria are adapted to low oxygen environments, but the mechanisms by which their growth in air is inhibited are not well understood. The citric acid cycle in the microaerophilic pathogen Campylobacter jejuni is potentially vulnerable, as it employs pyruvate and 2-oxoglutarate:acceptor oxidoreductases (Por and Oor), which contain labile (4Fe-4S) centres. Here, we show that both enzymes are rapidly inactivated after exposure of cells to a fully aerobic environment. We investigated the mechanisms that might protect enzyme activity and identify a role for the hemerythrin HerA (Cj0241). A herA mutant exhibits an aerobic growth defect and reduced Por and Oor activities after exposure to 21% (v/v) oxygen. Slow anaerobic recovery of these activities after oxygen damage was observed, but at similar rates in both wild-type and herA strains, suggesting the role of HerA is to prevent Fe-S cluster damage, rather than promote repair. Another hemerythrin (HerB; Cj1224) also plays a protective role. Purified HerA and HerB exhibited optical absorption, ligand binding and resonance Raman spectra typical of μ-oxo-bridged di-iron containing hemerythrins. We conclude that oxygen lability and poor repair of Por and Oor are major contributors to microaerophily in C.jejuni; hemerythrins help prevent enzyme damage microaerobically or during oxygen transients.

Original languageEnglish (US)
Pages (from-to)1105-1121
Number of pages17
JournalEnvironmental Microbiology
Volume16
Issue number4
DOIs
StatePublished - 2014

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Hemerythrin
Campylobacter jejuni
Sulfur
sulfur
Iron
iron
enzyme
Oxygen
Bacteria
oxygen
damage
bacterium
bacteria
Enzymes
enzymes
2-oxoglutarate synthase
repair
herb
herbs
Raman spectroscopy

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Hemerythrins in the microaerophilic bacterium Campylobacter jejuni help protect key iron-sulphur cluster enzymes from oxidative damage. / Kendall, John J.; Barrero-Tobon, Angelica M.; Hendrixson, David R.; Kelly, David J.

In: Environmental Microbiology, Vol. 16, No. 4, 2014, p. 1105-1121.

Research output: Contribution to journalArticle

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