Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells

Masao Yoshizumi, Hiroki Kurihara, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Yoshio Yazaki

Research output: Contribution to journalArticle

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Abstract

We have examined the effect of shear stress on the production of endothelin by cultured porcine endothelial cells. Low shear stress stimulated the expression of endothelin mRNA in polygonal endothelial cells with a peak time of 2 to 4 hours and also increased the release of immunoreactive endothelin into the culture medium. The expression of endothelin mRNA in the ellipsoidal endothelial cells under higher shear stress was not different from that of the control level. Our results suggest a possible role for hemodynamic shear stress in the regulation of endothelin production in vascular endothelial cells.

Original languageEnglish (US)
Pages (from-to)859-864
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
StatePublished - Jun 15 1989

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Endothelins
Endothelial cells
Hemodynamics
Shear stress
Cultured Cells
Endothelial Cells
Messenger RNA
Level control
Culture Media
Swine

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells. / Yoshizumi, Masao; Kurihara, Hiroki; Sugiyama, Takao; Takaku, Fumimaro; Yanagisawa, Masashi; Masaki, Tomoh; Yazaki, Yoshio.

In: Biochemical and Biophysical Research Communications, Vol. 161, No. 2, 15.06.1989, p. 859-864.

Research output: Contribution to journalArticle

Yoshizumi, Masao ; Kurihara, Hiroki ; Sugiyama, Takao ; Takaku, Fumimaro ; Yanagisawa, Masashi ; Masaki, Tomoh ; Yazaki, Yoshio. / Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 161, No. 2. pp. 859-864.
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