Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells

Masao Yoshizumi; Hiroki Kurihara; Takao Sugiyama; Fumimaro Takaku; Masashi Yanagisawa; Tomoh Masaki; Yoshio Yazaki

doi:10.1016/0006-291X(89)92679-X

Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells

Masao Yoshizumi, Hiroki Kurihara, Takao Sugiyama, Fumimaro Takaku, Masashi Yanagisawa, Tomoh Masaki, Yoshio Yazaki

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472 Scopus citations

Abstract

We have examined the effect of shear stress on the production of endothelin by cultured porcine endothelial cells. Low shear stress stimulated the expression of endothelin mRNA in polygonal endothelial cells with a peak time of 2 to 4 hours and also increased the release of immunoreactive endothelin into the culture medium. The expression of endothelin mRNA in the ellipsoidal endothelial cells under higher shear stress was not different from that of the control level. Our results suggest a possible role for hemodynamic shear stress in the regulation of endothelin production in vascular endothelial cells.

Original language	English (US)
Pages (from-to)	859-864
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	161
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(89)92679-X
State	Published - Jun 15 1989

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(89)92679-X

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title = "Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells",

abstract = "We have examined the effect of shear stress on the production of endothelin by cultured porcine endothelial cells. Low shear stress stimulated the expression of endothelin mRNA in polygonal endothelial cells with a peak time of 2 to 4 hours and also increased the release of immunoreactive endothelin into the culture medium. The expression of endothelin mRNA in the ellipsoidal endothelial cells under higher shear stress was not different from that of the control level. Our results suggest a possible role for hemodynamic shear stress in the regulation of endothelin production in vascular endothelial cells.",

author = "Masao Yoshizumi and Hiroki Kurihara and Takao Sugiyama and Fumimaro Takaku and Masashi Yanagisawa and Tomoh Masaki and Yoshio Yazaki",

note = "Funding Information: Am-S: This work was supported by a grant-in-aid for scientific research frcan the Ministry of Education, Science and Culture and by a srant from the Research Program on Cell Caicium Signals in the Cardi&ascular System, Japan.",

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T1 - Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells

AU - Yoshizumi, Masao

AU - Kurihara, Hiroki

AU - Sugiyama, Takao

AU - Takaku, Fumimaro

AU - Yanagisawa, Masashi

AU - Masaki, Tomoh

AU - Yazaki, Yoshio

N1 - Funding Information: Am-S: This work was supported by a grant-in-aid for scientific research frcan the Ministry of Education, Science and Culture and by a srant from the Research Program on Cell Caicium Signals in the Cardi&ascular System, Japan.

PY - 1989/6/15

Y1 - 1989/6/15

N2 - We have examined the effect of shear stress on the production of endothelin by cultured porcine endothelial cells. Low shear stress stimulated the expression of endothelin mRNA in polygonal endothelial cells with a peak time of 2 to 4 hours and also increased the release of immunoreactive endothelin into the culture medium. The expression of endothelin mRNA in the ellipsoidal endothelial cells under higher shear stress was not different from that of the control level. Our results suggest a possible role for hemodynamic shear stress in the regulation of endothelin production in vascular endothelial cells.

AB - We have examined the effect of shear stress on the production of endothelin by cultured porcine endothelial cells. Low shear stress stimulated the expression of endothelin mRNA in polygonal endothelial cells with a peak time of 2 to 4 hours and also increased the release of immunoreactive endothelin into the culture medium. The expression of endothelin mRNA in the ellipsoidal endothelial cells under higher shear stress was not different from that of the control level. Our results suggest a possible role for hemodynamic shear stress in the regulation of endothelin production in vascular endothelial cells.

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Hemodynamic shear stress stimulates endothelin production by cultured endothelial cells

Abstract

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