Hepatic collagen proline hydroxylase activity in alcoholic hepatitis: effect of d-penicillamine

Esteban Mezey, James J. Potter, Frank L. Iber, Willis C. Maddrey

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Hepatic collagen proline hydroxylase activity was measured in the presence of Triton X-100 in 80 patients with alcoholic liver disease. The mean enzyme activity was elevated only in patients with alcoholic hepatitis and not in those with fatty infiltration or inactive cirrhosis. Elevations of the urinary excretion of peptide-bound hydroxyproline and glycosaminoglycans were found in patients with alcoholic hepatitis and inactive cirrhosis. In patients with alcoholic hepatitis with initial elevation of enzymes values (greater than the mean control value plus 2 S.D., >171 dpm/mg of protein per minute), there was a further increase in the enzyme in four of five patients receiving placebo, but a fall in all three receiving d-penicillamine, 1.0 gm/day, for a period of 1 month. However, no significant changes were found in the mean activity of the enzyme in the entire group of patients with alcoholic hepatitis after the administration of either placebo or d-penicillamine. The urinary excretion of peptide-bound hydroxyproline decreased in patients receiving placebo but not in those receiving d-penicillamine; the urinary excretion of glycosaminoglycans was not changed by either treatment. These studies show that among alcoholic patients with liver disease, those with alcoholic hepatitis have the greatest changes in parameters of collagen metabolism. In patients with alcoholic hepatitis, d-penicillamine appears to suppress elevated values of collagen proline hydroxylase while preventing decreases in the urinary excretion of peptide-bound hydroxyproline found with the administration of placebo. This suggests that d-penicillamine may decrease augmented hepatic collagen synthesis.

Original languageEnglish (US)
Pages (from-to)92-100
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume93
Issue number1
StatePublished - 1979

Fingerprint

Prolyl Hydroxylases
Alcoholic Hepatitis
Penicillamine
Collagen
Hydroxyproline
Liver
Glycosaminoglycans
Peptides
Placebos
Enzymes
Octoxynol
Enzyme activity
Infiltration
Metabolism
Fibrosis
Alcoholic Liver Diseases
Alcoholics
Liver Diseases
Proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Hepatic collagen proline hydroxylase activity in alcoholic hepatitis : effect of d-penicillamine. / Mezey, Esteban; Potter, James J.; Iber, Frank L.; Maddrey, Willis C.

In: The Journal of Laboratory and Clinical Medicine, Vol. 93, No. 1, 1979, p. 92-100.

Research output: Contribution to journalArticle

@article{2c702edea39e4f15a1fa8fffeb42fa0f,
title = "Hepatic collagen proline hydroxylase activity in alcoholic hepatitis: effect of d-penicillamine",
abstract = "Hepatic collagen proline hydroxylase activity was measured in the presence of Triton X-100 in 80 patients with alcoholic liver disease. The mean enzyme activity was elevated only in patients with alcoholic hepatitis and not in those with fatty infiltration or inactive cirrhosis. Elevations of the urinary excretion of peptide-bound hydroxyproline and glycosaminoglycans were found in patients with alcoholic hepatitis and inactive cirrhosis. In patients with alcoholic hepatitis with initial elevation of enzymes values (greater than the mean control value plus 2 S.D., >171 dpm/mg of protein per minute), there was a further increase in the enzyme in four of five patients receiving placebo, but a fall in all three receiving d-penicillamine, 1.0 gm/day, for a period of 1 month. However, no significant changes were found in the mean activity of the enzyme in the entire group of patients with alcoholic hepatitis after the administration of either placebo or d-penicillamine. The urinary excretion of peptide-bound hydroxyproline decreased in patients receiving placebo but not in those receiving d-penicillamine; the urinary excretion of glycosaminoglycans was not changed by either treatment. These studies show that among alcoholic patients with liver disease, those with alcoholic hepatitis have the greatest changes in parameters of collagen metabolism. In patients with alcoholic hepatitis, d-penicillamine appears to suppress elevated values of collagen proline hydroxylase while preventing decreases in the urinary excretion of peptide-bound hydroxyproline found with the administration of placebo. This suggests that d-penicillamine may decrease augmented hepatic collagen synthesis.",
author = "Esteban Mezey and Potter, {James J.} and Iber, {Frank L.} and Maddrey, {Willis C.}",
year = "1979",
language = "English (US)",
volume = "93",
pages = "92--100",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Hepatic collagen proline hydroxylase activity in alcoholic hepatitis

T2 - effect of d-penicillamine

AU - Mezey, Esteban

AU - Potter, James J.

AU - Iber, Frank L.

AU - Maddrey, Willis C.

PY - 1979

Y1 - 1979

N2 - Hepatic collagen proline hydroxylase activity was measured in the presence of Triton X-100 in 80 patients with alcoholic liver disease. The mean enzyme activity was elevated only in patients with alcoholic hepatitis and not in those with fatty infiltration or inactive cirrhosis. Elevations of the urinary excretion of peptide-bound hydroxyproline and glycosaminoglycans were found in patients with alcoholic hepatitis and inactive cirrhosis. In patients with alcoholic hepatitis with initial elevation of enzymes values (greater than the mean control value plus 2 S.D., >171 dpm/mg of protein per minute), there was a further increase in the enzyme in four of five patients receiving placebo, but a fall in all three receiving d-penicillamine, 1.0 gm/day, for a period of 1 month. However, no significant changes were found in the mean activity of the enzyme in the entire group of patients with alcoholic hepatitis after the administration of either placebo or d-penicillamine. The urinary excretion of peptide-bound hydroxyproline decreased in patients receiving placebo but not in those receiving d-penicillamine; the urinary excretion of glycosaminoglycans was not changed by either treatment. These studies show that among alcoholic patients with liver disease, those with alcoholic hepatitis have the greatest changes in parameters of collagen metabolism. In patients with alcoholic hepatitis, d-penicillamine appears to suppress elevated values of collagen proline hydroxylase while preventing decreases in the urinary excretion of peptide-bound hydroxyproline found with the administration of placebo. This suggests that d-penicillamine may decrease augmented hepatic collagen synthesis.

AB - Hepatic collagen proline hydroxylase activity was measured in the presence of Triton X-100 in 80 patients with alcoholic liver disease. The mean enzyme activity was elevated only in patients with alcoholic hepatitis and not in those with fatty infiltration or inactive cirrhosis. Elevations of the urinary excretion of peptide-bound hydroxyproline and glycosaminoglycans were found in patients with alcoholic hepatitis and inactive cirrhosis. In patients with alcoholic hepatitis with initial elevation of enzymes values (greater than the mean control value plus 2 S.D., >171 dpm/mg of protein per minute), there was a further increase in the enzyme in four of five patients receiving placebo, but a fall in all three receiving d-penicillamine, 1.0 gm/day, for a period of 1 month. However, no significant changes were found in the mean activity of the enzyme in the entire group of patients with alcoholic hepatitis after the administration of either placebo or d-penicillamine. The urinary excretion of peptide-bound hydroxyproline decreased in patients receiving placebo but not in those receiving d-penicillamine; the urinary excretion of glycosaminoglycans was not changed by either treatment. These studies show that among alcoholic patients with liver disease, those with alcoholic hepatitis have the greatest changes in parameters of collagen metabolism. In patients with alcoholic hepatitis, d-penicillamine appears to suppress elevated values of collagen proline hydroxylase while preventing decreases in the urinary excretion of peptide-bound hydroxyproline found with the administration of placebo. This suggests that d-penicillamine may decrease augmented hepatic collagen synthesis.

UR - http://www.scopus.com/inward/record.url?scp=0018409102&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018409102&partnerID=8YFLogxK

M3 - Article

C2 - 215682

AN - SCOPUS:0018409102

VL - 93

SP - 92

EP - 100

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 1

ER -