Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay

J. D. McGarry, M. J. Stark, D. W. Foster

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

A simple radioisotopic assay for malonyl-CoA is described. The method is based on the malonyl-CoA-dependent incorporation of labeled acetyl-CoA into palmitic acid catalyzed by fatty acid synthetase in the presence of NADPH. Its main advantages over the more conventional spectrophotometric procedure is that it is extremely sensitive and allows the simultaneous determination of picomole quantities of malonyl-CoA in multiple tissue extracts. It should prove particularly suitable for studies on the regulation of lipid metabolism in isolated hepatocytes where the quantity of tissue available for analysis is frequently very small. Application of the method to the measurement of malonyl-CoA in livers from fed, fasted, and diabetic rats yielded values that were consistent with the recently postulated role of malonyl-CoA in the regulation of hepatic ketone body production.

Original languageEnglish (US)
Pages (from-to)8291-8293
Number of pages3
JournalJournal of Biological Chemistry
Volume253
Issue number22
StatePublished - 1978

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Malonyl Coenzyme A
Rats
Assays
Liver
Fatty Acid Synthases
Ketone Bodies
Acetyl Coenzyme A
Tissue Extracts
Palmitic Acid
NADP
Lipid Metabolism
Hepatocytes
Tissue

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hepatic malonyl-CoA levels of fed, fasted and diabetic rats as measured using a simple radioisotopic assay. / McGarry, J. D.; Stark, M. J.; Foster, D. W.

In: Journal of Biological Chemistry, Vol. 253, No. 22, 1978, p. 8291-8293.

Research output: Contribution to journalArticle

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