Heterocoagulation as a facile route to prepare stable serum albumin-nanoparticle conjugates for biomedical applications: Synthetic protocols and mechanistic insights

Kin Man Au, Steven P. Armes

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

There is increasing interest in using serum albumin, the most abundant plasma protein, as a stabilizing agent in the context of nanomedicine. Using poly(vinyl amine)-stabilized polypyrrole nanoparticles as an example, we report a facile generic route to prepare serum albumin-nanoparticle conjugates via heterocoagulation. Time-resolved dynamic light scattering (DLS), disk centrifuge photosedimentometry (DCP), and circular dichroism (CD) spectroscopy studies confirm that bovine serum albumin (BSA) adsorbs rapidly onto the cationic poly(vinyl amine)-stabilized polypyrrole nanoparticles and suggest that the initial well-defined protein coronal is subsequently cross-linked via thiol-disulfide exchange. These BSA-nanoparticle conjugates were further characterized by X-ray photoelectron spectroscopy (XPS), aqueous electrophoresis, field emission scanning electron microscopy (FE SEM), and transmission electron microscopy (TEM). They exhibit excellent long-term colloidal stability under physiological conditions without further purification, suggesting strong irreversible adsorption by the BSA. Protein adsorption appears to be co-operative and both thermodynamic and mechanistic aspects were examined via aqueous electrophoresis, DCP, and DLS studies.

Original languageEnglish (US)
Pages (from-to)8261-8279
Number of pages19
JournalACS Nano
Volume6
Issue number9
DOIs
StatePublished - Sep 25 2012
Externally publishedYes

Keywords

  • heterocoagulation
  • polypyrrole nanoparticles
  • protein adsorption
  • protein-nanoparticle interactions
  • serum albumin

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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