Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines

N. W. Toribara, T. L. Sack, J. R. Gum, S. B. Ho, J. E. Shively, J. K V Willson, Y. S. Kim

Research output: Contribution to journalArticle

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Abstract

Sodium butyrate induces morphological and biochemical changes consistent with a more differentiated phenotype in some colon cancer cell lines. These changes include increased expression of carcinoembryonic antigen (CEA) and other oncodevelopmental markers. We utilized domain-specific probes and polyclonal antibodies against CEA-related antigens to study sodium butyrate-induced expression of the CEA gene family in a villous adenoma-derived cell line, which is nontumorigenic in nude mice (VACO 235), and two colonic carcinoma cell lines known to respond to sodium butyrate exposure by phenotypic differentiation (HT-29 and LS 174T). The induction begins as quickly as 24 h after exposure and occurs primarily at a transcriptional level, although some translational control is also evident. No evidence was found for gene amplification, rearrangement, or methylation to account for the mechanism of this transcriptional control. [35S]Cysteine pulse-labeled cell lysate immunoblots and polyadenylated RNA blot hybridization suggest that increases in mRNA transcript and CEA-related glycoprotein levels are primarily due to increased synthesis rather than decreased degradation. A considerable amount of heterogeneity is seen in the biosynthesis of the CEA-related glycoproteins, with each cell line showing a distinct pattern of CEA-related antigen expression from a limited number of mRNA transcripts.

Original languageEnglish (US)
Pages (from-to)3321-3327
Number of pages7
JournalCancer Research
Volume49
Issue number12
StatePublished - 1989

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Carcinoembryonic Antigen
Colonic Neoplasms
Antigens
Cell Line
Butyric Acid
Messenger RNA
Glycoproteins
Villous Adenoma
Gene Rearrangement
Gene Amplification
Nude Mice
Methylation
Cysteine
Carcinoma
Phenotype
Antibodies
Genes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Toribara, N. W., Sack, T. L., Gum, J. R., Ho, S. B., Shively, J. E., Willson, J. K. V., & Kim, Y. S. (1989). Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. Cancer Research, 49(12), 3321-3327.

Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. / Toribara, N. W.; Sack, T. L.; Gum, J. R.; Ho, S. B.; Shively, J. E.; Willson, J. K V; Kim, Y. S.

In: Cancer Research, Vol. 49, No. 12, 1989, p. 3321-3327.

Research output: Contribution to journalArticle

Toribara, NW, Sack, TL, Gum, JR, Ho, SB, Shively, JE, Willson, JKV & Kim, YS 1989, 'Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines', Cancer Research, vol. 49, no. 12, pp. 3321-3327.
Toribara NW, Sack TL, Gum JR, Ho SB, Shively JE, Willson JKV et al. Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. Cancer Research. 1989;49(12):3321-3327.
Toribara, N. W. ; Sack, T. L. ; Gum, J. R. ; Ho, S. B. ; Shively, J. E. ; Willson, J. K V ; Kim, Y. S. / Heterogeneity in the induction and expression of carcinoembryonic antigen-related antigens in human colon cancer cell lines. In: Cancer Research. 1989 ; Vol. 49, No. 12. pp. 3321-3327.
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