Heterogeneity of B cell clones: Immunoglobulin-secreting subsets

J. Kettman, A. Soederberg, I. Lefkovits

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Single murine B cells can be induced to grow clonally by a combination of mitogens (dextran sulfate and LPS) and the presence of appropriate accessory cells. We show here that irradiated cells of a tissue culture cell line (WEHI-3) can substitute for naturally occurring adherent cells. Single-cell growth as well as Ig secretion are induced by irradiated cells of WEHI-3. There are no histocompatibility restrictions between the cells of the accessory cell line and the responding B cells. Under limiting dilution conditions, dextran sulfate and LPS act synergistically in inducing both growth and Ig secretion in these WEHI-3-dependent cultures. Although Ig synthesis occurs only in cultures where B-cell growth occurs, not all clones of B cells produce Ig. This is apparently not related to the clone size or to the number of initiated precursor cells; instead mitogen-induced growing B-cell clones seem to be heterogeneous with respect to Ig production.

Original languageEnglish (US)
Pages (from-to)70-77
Number of pages8
JournalCellular Immunology
Volume66
Issue number1
DOIs
StatePublished - Jan 1 1982

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Immunoglobulins
B-Lymphocytes
Clone Cells
Dextran Sulfate
Mitogens
Growth
Cell Line
Histocompatibility

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Heterogeneity of B cell clones : Immunoglobulin-secreting subsets. / Kettman, J.; Soederberg, A.; Lefkovits, I.

In: Cellular Immunology, Vol. 66, No. 1, 01.01.1982, p. 70-77.

Research output: Contribution to journalArticle

Kettman, J. ; Soederberg, A. ; Lefkovits, I. / Heterogeneity of B cell clones : Immunoglobulin-secreting subsets. In: Cellular Immunology. 1982 ; Vol. 66, No. 1. pp. 70-77.
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