Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring in hypertrophic cardiomyopathy

Tetsuo Konno, Dan Chen, Libin Wang, Hiroko Wakimoto, Polakit Teekakirikul, Matthew Nayor, Masataka Kawana, Seda Eminaga, Joshua M. Gorham, Kumar Pandya, Oliver Smithies, Francisco J. Naya, Eric N. Olson, J. G. Seidman, Christine E. Seidman

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Abstract

Unknown molecular responses to sarcomere protein gene mutations account for pathologic remodeling in hypertrophic cardiomyopathy (HCM), producing myocyte growth and increased cardiac fibrosis. To determine if hypertrophic signals activated myocyte enhancer factor-2 (Mef2), we studied mice carrying the HCM mutation, myosin heavy-chain Arg403Gln, (MHC403/+) and an Mef2-dependent β-galactosidase reporter transgene. In young, prehypertrophic MHC403/+ mice the reporter was not activated. In hypertrophic hearts, activation of the Mef2-dependent reporter was remarkably heterogeneous and was observed consistently in myocytes that bordered fibrotic foci with necrotic cells, MHC403/+ myocytes with Mef2-dependent reporter activation reexpressed the fetal myosin isoform (βMHC), a molecular marker of hypertrophy, although MHC403/+ myocytes with or without βMHC expression were comparably enlarged over WT myocytes. To consider Mef2 roles in severe HCM, we studied homozygous MHC403/403 mice, which have accelerated remodeling, widespread myocyte necrosis, and neonatal lethality. Levels of phosphorylated class II histone deacetylases that activate Mef2 were substantially increased in MHC403/403 hearts, but Mef2-dependent reporter activation was patchy. Sequential analyses showed myocytes increased Mef2-dependent reporter activity before death. Our data dissociate myocyte hypertrophy, a consistent response in HCM, from heterogeneous Mef2 activation and reexpression of a fetal gene program. The temporal and spatial relationship of Mef2-dependent gene activation with myocyte necrosis and fibrosis in MHC403/+ and MHC403/403 hearts defines Mef2 activation as a molecular signature of stressed HCM myocytes that are poised to die.

Original languageEnglish (US)
Pages (from-to)18097-18102
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number42
DOIs
StatePublished - Oct 19 2010

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MEF2 Transcription Factors
Hypertrophic Cardiomyopathy
Muscle Cells
Cicatrix
Hypertrophy
Fibrosis
Necrosis
Galactosidases
Sarcomeres
Mutation
Histone Deacetylases
Myosin Heavy Chains
Myosins
Transgenes
Transcriptional Activation

Keywords

  • Fibrosis
  • Hypertrophy
  • Sarcomere protein gene mutation

ASJC Scopus subject areas

  • General

Cite this

Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring in hypertrophic cardiomyopathy. / Konno, Tetsuo; Chen, Dan; Wang, Libin; Wakimoto, Hiroko; Teekakirikul, Polakit; Nayor, Matthew; Kawana, Masataka; Eminaga, Seda; Gorham, Joshua M.; Pandya, Kumar; Smithies, Oliver; Naya, Francisco J.; Olson, Eric N.; Seidman, J. G.; Seidman, Christine E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 42, 19.10.2010, p. 18097-18102.

Research output: Contribution to journalArticle

Konno, T, Chen, D, Wang, L, Wakimoto, H, Teekakirikul, P, Nayor, M, Kawana, M, Eminaga, S, Gorham, JM, Pandya, K, Smithies, O, Naya, FJ, Olson, EN, Seidman, JG & Seidman, CE 2010, 'Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring in hypertrophic cardiomyopathy', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 42, pp. 18097-18102. https://doi.org/10.1073/pnas.1012826107
Konno, Tetsuo ; Chen, Dan ; Wang, Libin ; Wakimoto, Hiroko ; Teekakirikul, Polakit ; Nayor, Matthew ; Kawana, Masataka ; Eminaga, Seda ; Gorham, Joshua M. ; Pandya, Kumar ; Smithies, Oliver ; Naya, Francisco J. ; Olson, Eric N. ; Seidman, J. G. ; Seidman, Christine E. / Heterogeneous myocyte enhancer factor-2 (Mef2) activation in myocytes predicts focal scarring in hypertrophic cardiomyopathy. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 42. pp. 18097-18102.
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AU - Konno, Tetsuo

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AU - Teekakirikul, Polakit

AU - Nayor, Matthew

AU - Kawana, Masataka

AU - Eminaga, Seda

AU - Gorham, Joshua M.

AU - Pandya, Kumar

AU - Smithies, Oliver

AU - Naya, Francisco J.

AU - Olson, Eric N.

AU - Seidman, J. G.

AU - Seidman, Christine E.

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N2 - Unknown molecular responses to sarcomere protein gene mutations account for pathologic remodeling in hypertrophic cardiomyopathy (HCM), producing myocyte growth and increased cardiac fibrosis. To determine if hypertrophic signals activated myocyte enhancer factor-2 (Mef2), we studied mice carrying the HCM mutation, myosin heavy-chain Arg403Gln, (MHC403/+) and an Mef2-dependent β-galactosidase reporter transgene. In young, prehypertrophic MHC403/+ mice the reporter was not activated. In hypertrophic hearts, activation of the Mef2-dependent reporter was remarkably heterogeneous and was observed consistently in myocytes that bordered fibrotic foci with necrotic cells, MHC403/+ myocytes with Mef2-dependent reporter activation reexpressed the fetal myosin isoform (βMHC), a molecular marker of hypertrophy, although MHC403/+ myocytes with or without βMHC expression were comparably enlarged over WT myocytes. To consider Mef2 roles in severe HCM, we studied homozygous MHC403/403 mice, which have accelerated remodeling, widespread myocyte necrosis, and neonatal lethality. Levels of phosphorylated class II histone deacetylases that activate Mef2 were substantially increased in MHC403/403 hearts, but Mef2-dependent reporter activation was patchy. Sequential analyses showed myocytes increased Mef2-dependent reporter activity before death. Our data dissociate myocyte hypertrophy, a consistent response in HCM, from heterogeneous Mef2 activation and reexpression of a fetal gene program. The temporal and spatial relationship of Mef2-dependent gene activation with myocyte necrosis and fibrosis in MHC403/+ and MHC403/403 hearts defines Mef2 activation as a molecular signature of stressed HCM myocytes that are poised to die.

AB - Unknown molecular responses to sarcomere protein gene mutations account for pathologic remodeling in hypertrophic cardiomyopathy (HCM), producing myocyte growth and increased cardiac fibrosis. To determine if hypertrophic signals activated myocyte enhancer factor-2 (Mef2), we studied mice carrying the HCM mutation, myosin heavy-chain Arg403Gln, (MHC403/+) and an Mef2-dependent β-galactosidase reporter transgene. In young, prehypertrophic MHC403/+ mice the reporter was not activated. In hypertrophic hearts, activation of the Mef2-dependent reporter was remarkably heterogeneous and was observed consistently in myocytes that bordered fibrotic foci with necrotic cells, MHC403/+ myocytes with Mef2-dependent reporter activation reexpressed the fetal myosin isoform (βMHC), a molecular marker of hypertrophy, although MHC403/+ myocytes with or without βMHC expression were comparably enlarged over WT myocytes. To consider Mef2 roles in severe HCM, we studied homozygous MHC403/403 mice, which have accelerated remodeling, widespread myocyte necrosis, and neonatal lethality. Levels of phosphorylated class II histone deacetylases that activate Mef2 were substantially increased in MHC403/403 hearts, but Mef2-dependent reporter activation was patchy. Sequential analyses showed myocytes increased Mef2-dependent reporter activity before death. Our data dissociate myocyte hypertrophy, a consistent response in HCM, from heterogeneous Mef2 activation and reexpression of a fetal gene program. The temporal and spatial relationship of Mef2-dependent gene activation with myocyte necrosis and fibrosis in MHC403/+ and MHC403/403 hearts defines Mef2 activation as a molecular signature of stressed HCM myocytes that are poised to die.

KW - Fibrosis

KW - Hypertrophy

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