Heterotransplantation of small-cell carcinoma of the lung into nude mice

Comparison of intracranial and subcutaneous routes

A. F. Gazdar, D. N. Carney, H. L. Sims, A. Simmons

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The authors compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small-cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types and specimens were similar, but s.c. and i.c. transplants differed. S.C. tumors had longer latent times, were non-invasive and non-lethal. I.c. tumors had shorter latent periods, invariably grew in the meninges, frequently invaded and destroyed the underlying brain, and were lethal. The tumor-inducing dose for i.c. transplantation was 10 to 1,000 times lower than for s.c. transplantation. Pooled colonies of SCCL tumor specimens grown in soft agarose were inoculated i.c. While they contained relatively small numbers of cells (400-10,000), 83% of these colony specimens induced tumors after 58-243 days, confirming the 'stem-cell' origin of the colonies. I.c. and s.c. transplants retained the characteristic morphology of SCCL, and, with one exception, did not metastasize to distant organs. Continuous cell lines could be established readily from both types of transplants, and they retained the characteristic cytology, growth and biochemical properties of the original SCCL tumors. I.c. heterotransplantation of SCCL is a useful tool, especially when small numbers of tumor cells are available, and may provide a model to study the biology and therapy of meningeal carcinomatosis.

Original languageEnglish (US)
Pages (from-to)777-783
Number of pages7
JournalInternational Journal of Cancer
Volume28
Issue number6
StatePublished - 1981

Fingerprint

Small Cell Lung Carcinoma
Nude Mice
Neoplasms
Transplants
Sepharose
Cell Count
Transplantation
Meningeal Carcinomatosis
Cell Line
Meninges
Cell Biology
Stem Cells

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Heterotransplantation of small-cell carcinoma of the lung into nude mice : Comparison of intracranial and subcutaneous routes. / Gazdar, A. F.; Carney, D. N.; Sims, H. L.; Simmons, A.

In: International Journal of Cancer, Vol. 28, No. 6, 1981, p. 777-783.

Research output: Contribution to journalArticle

@article{f7adb7b521eb403fa4b9e73827470da2,
title = "Heterotransplantation of small-cell carcinoma of the lung into nude mice: Comparison of intracranial and subcutaneous routes",
abstract = "The authors compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small-cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types and specimens were similar, but s.c. and i.c. transplants differed. S.C. tumors had longer latent times, were non-invasive and non-lethal. I.c. tumors had shorter latent periods, invariably grew in the meninges, frequently invaded and destroyed the underlying brain, and were lethal. The tumor-inducing dose for i.c. transplantation was 10 to 1,000 times lower than for s.c. transplantation. Pooled colonies of SCCL tumor specimens grown in soft agarose were inoculated i.c. While they contained relatively small numbers of cells (400-10,000), 83{\%} of these colony specimens induced tumors after 58-243 days, confirming the 'stem-cell' origin of the colonies. I.c. and s.c. transplants retained the characteristic morphology of SCCL, and, with one exception, did not metastasize to distant organs. Continuous cell lines could be established readily from both types of transplants, and they retained the characteristic cytology, growth and biochemical properties of the original SCCL tumors. I.c. heterotransplantation of SCCL is a useful tool, especially when small numbers of tumor cells are available, and may provide a model to study the biology and therapy of meningeal carcinomatosis.",
author = "Gazdar, {A. F.} and Carney, {D. N.} and Sims, {H. L.} and A. Simmons",
year = "1981",
language = "English (US)",
volume = "28",
pages = "777--783",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Heterotransplantation of small-cell carcinoma of the lung into nude mice

T2 - Comparison of intracranial and subcutaneous routes

AU - Gazdar, A. F.

AU - Carney, D. N.

AU - Sims, H. L.

AU - Simmons, A.

PY - 1981

Y1 - 1981

N2 - The authors compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small-cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types and specimens were similar, but s.c. and i.c. transplants differed. S.C. tumors had longer latent times, were non-invasive and non-lethal. I.c. tumors had shorter latent periods, invariably grew in the meninges, frequently invaded and destroyed the underlying brain, and were lethal. The tumor-inducing dose for i.c. transplantation was 10 to 1,000 times lower than for s.c. transplantation. Pooled colonies of SCCL tumor specimens grown in soft agarose were inoculated i.c. While they contained relatively small numbers of cells (400-10,000), 83% of these colony specimens induced tumors after 58-243 days, confirming the 'stem-cell' origin of the colonies. I.c. and s.c. transplants retained the characteristic morphology of SCCL, and, with one exception, did not metastasize to distant organs. Continuous cell lines could be established readily from both types of transplants, and they retained the characteristic cytology, growth and biochemical properties of the original SCCL tumors. I.c. heterotransplantation of SCCL is a useful tool, especially when small numbers of tumor cells are available, and may provide a model to study the biology and therapy of meningeal carcinomatosis.

AB - The authors compared and contrasted intracranial (i.c.) and subcutaneous (s.c.) heterotransplantation of small-cell carcinoma of the lung (SCCL) into athymic nude mice. Fresh human SCCL tumor specimens, tumor colonies grown in soft agarose and continuous cell lines were used. Tumors induced by the three types and specimens were similar, but s.c. and i.c. transplants differed. S.C. tumors had longer latent times, were non-invasive and non-lethal. I.c. tumors had shorter latent periods, invariably grew in the meninges, frequently invaded and destroyed the underlying brain, and were lethal. The tumor-inducing dose for i.c. transplantation was 10 to 1,000 times lower than for s.c. transplantation. Pooled colonies of SCCL tumor specimens grown in soft agarose were inoculated i.c. While they contained relatively small numbers of cells (400-10,000), 83% of these colony specimens induced tumors after 58-243 days, confirming the 'stem-cell' origin of the colonies. I.c. and s.c. transplants retained the characteristic morphology of SCCL, and, with one exception, did not metastasize to distant organs. Continuous cell lines could be established readily from both types of transplants, and they retained the characteristic cytology, growth and biochemical properties of the original SCCL tumors. I.c. heterotransplantation of SCCL is a useful tool, especially when small numbers of tumor cells are available, and may provide a model to study the biology and therapy of meningeal carcinomatosis.

UR - http://www.scopus.com/inward/record.url?scp=0019847006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019847006&partnerID=8YFLogxK

M3 - Article

VL - 28

SP - 777

EP - 783

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6

ER -