Hid arbitrates collective cell death in the Drosophila wing

Gianella Garcia-Hughes, Nichole Link, Anwesha B. Ghosh, John M. Abrams

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Elimination of cells and tissues by apoptosis is a highly conserved and tightly regulated process. In Drosophila, the entire wing epithelium is completely removed shortly after eclosion. The cells that make up this epithelium are collectively eliminated through a highly synchronized form of apoptotic cell death, involving canonical apoptosome genes. Here we present evidence that collective cell death does not require cell-cell contact and show that transcription of the IAP antagonist, head involution defective, is acutely induced in wing epithelial cells prior to this process. hid mRNAs accumulate to levels that exceed a component of the ribosome and likewise, Hid protein becomes highly abundant in these same cells. hid function is required for collective cell death, since loss of function mutants shows persisting wing epithelial cells and, furthermore, silencing of the hormone bursicon in the CNS produced collective cell death defective phenotypes manifested in the wing epithelium. Taken together, our observations suggest that acute induction of Hid primes wing epithelial cells for collective cell death and that Bursicon is a strong candidate to trigger this process, possibly by activating the abundant pool of Hid protein already present.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalMechanisms of Development
Volume138
DOIs
StatePublished - Nov 1 2015

Fingerprint

Drosophila
Cell Death
Epithelium
Epithelial Cells
Apoptosomes
Ribosomes
Proteins
Head
Hormones
Apoptosis
Phenotype
Messenger RNA
Genes
bursicon

Keywords

  • Apoptosis
  • Collective cell death
  • Development
  • Eclosion
  • Hormone

ASJC Scopus subject areas

  • Developmental Biology
  • Embryology

Cite this

Hid arbitrates collective cell death in the Drosophila wing. / Garcia-Hughes, Gianella; Link, Nichole; Ghosh, Anwesha B.; Abrams, John M.

In: Mechanisms of Development, Vol. 138, 01.11.2015, p. 349-355.

Research output: Contribution to journalArticle

Garcia-Hughes, Gianella ; Link, Nichole ; Ghosh, Anwesha B. ; Abrams, John M. / Hid arbitrates collective cell death in the Drosophila wing. In: Mechanisms of Development. 2015 ; Vol. 138. pp. 349-355.
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