High-level expression of B7-H1 molecules by dendritic cells suppresses the function of activated T cells and desensitizes allergen-primed animals

Kyung Kim Hee, Hongbing Guan, Guorui Zu, Hui Li, Lizhi Wu, Xu Feng, Craig Elmets, Yangxin Fu, Hui Xu

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

A body of evidence indicates that expression of the programmed cell death 1 (PD-1) receptor by activated T cells plays an important role in the down-regulation of immune responses; however, the functions of its known ligands, B7-H1 (PD-L1) and B7-dendritic cell (DC; PD-L2), at the effector phase of immune responses are less clear. In the current study, we investigated the roles of B7-H1 in DC-mediated regulation of hapten-activated T cells and the delayed-type contact hypersensitivity response in primed animals. We found that the expression of B7-H1 and B7-DC was induced on activation of DC by hapten stimulation. Blockade of B7-H1, but not B7-DC, enhanced the activity of hapten-specific T cells. Interaction with a DC line that expresses high cell-surface levels of B7-H1 (B7-H1/DC) suppressed the proliferation of, and cytokine production by, activated T cells. In vivo administration of hapten-carrying B7-H1/DC desensitized the response of sensitized animals to hapten challenge, and this desensitization was hapten-specific. These data indicate that B7-H1 expressed by DC mediates inhibitory signals for activated T cells and suppresses the elicitation of immune responses. The ability of B7-H1/DC to inhibit the function of preactivated T cells in vivo suggests novel strategies for the treatment of immune response-mediated disorders.

Original languageEnglish (US)
Pages (from-to)686-695
Number of pages10
JournalJournal of Leukocyte Biology
Volume79
Issue number4
DOIs
StatePublished - Apr 1 2006

Fingerprint

B7 Antigens
Haptens
Allergens
Dendritic Cells
T-Lymphocytes
Programmed Cell Death 1 Receptor
Contact Dermatitis
Delayed Hypersensitivity
Down-Regulation
Cytokines
Ligands

Keywords

  • Contact hypersensitivity
  • Cytokines
  • Immunology
  • PD-1

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

High-level expression of B7-H1 molecules by dendritic cells suppresses the function of activated T cells and desensitizes allergen-primed animals. / Hee, Kyung Kim; Guan, Hongbing; Zu, Guorui; Li, Hui; Wu, Lizhi; Feng, Xu; Elmets, Craig; Fu, Yangxin; Xu, Hui.

In: Journal of Leukocyte Biology, Vol. 79, No. 4, 01.04.2006, p. 686-695.

Research output: Contribution to journalArticle

Hee, Kyung Kim ; Guan, Hongbing ; Zu, Guorui ; Li, Hui ; Wu, Lizhi ; Feng, Xu ; Elmets, Craig ; Fu, Yangxin ; Xu, Hui. / High-level expression of B7-H1 molecules by dendritic cells suppresses the function of activated T cells and desensitizes allergen-primed animals. In: Journal of Leukocyte Biology. 2006 ; Vol. 79, No. 4. pp. 686-695.
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