High levels of in vitro IgM rheumatoid factor synthesis correlate with HLA-DR4 in normal individuals

N. J. Olsen, P. Stastny, H. E. Jasin

Research output: Contribution to journalArticle

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Abstract

The association between the HLA-DR4 histocompatibility antigen and in vitro synthesis of IgM and IgM rheumatoid factor (IgM-RF) by blood mononuclear cells was investigated in 35 normal subjects. In vitro cultures of T and B cells were stimulated with pokeweed mitogen, and the secreted IgM-RF, tetanus antibody, and total IgM protein were measured by solid-phase radioimmunoassay. In cultures containing unseparated T cells, IgM-RF production in the DR4+ and DR4- subgroups was not significantly different. However, depletion of OKT8+ cells containing T suppressor cells resulted in significantly higher IgM and IgM-RF synthesis in the DR4+ subgroup. Moreover, 6 of the 8 highest levels of IgM-RF were produced by DR4+ individuals, while only 7 of the remaining 27 individuals were DR4+ (P = 0.035). Ratios of secreted IgM-RF: IgM indicated that there was a relative enrichment for IgM-RF-specific B cell precursors in DR4+ high responders, although total numbers of circulating B cells were not increased. High responder B cells had high levels of responsiveness when mixed with T helper cells from low responders, whereas low responder B cells consistently produced low responses, even when cocultured with T helper cells from high responder donors. The data suggest that a subset of normal individuals has a pokeweed mitogen-responsive lymphocyte population that contains a B cell subpopulation specific for IgM-RF synthesis and that this condition is associated with HLA-DR4. In certain individuals who have the DR4 type, there may be a component of a susceptible genetic background, upon which other factors act to induce IgM-RF synthesis and which may produce clinical manifestations of rheumatoid arthritis.

Original languageEnglish (US)
Pages (from-to)841-848
Number of pages8
JournalArthritis and Rheumatism
Volume30
Issue number8
StatePublished - 1987

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HLA-DR4 Antigen
Rheumatoid Factor
Immunoglobulin M
B-Lymphocytes
Pokeweed Mitogens
Helper-Inducer T-Lymphocytes
In Vitro Techniques
T-Lymphocytes
Histocompatibility Antigens
B-Lymphoid Precursor Cells
Tetanus

ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

High levels of in vitro IgM rheumatoid factor synthesis correlate with HLA-DR4 in normal individuals. / Olsen, N. J.; Stastny, P.; Jasin, H. E.

In: Arthritis and Rheumatism, Vol. 30, No. 8, 1987, p. 841-848.

Research output: Contribution to journalArticle

Olsen, N. J. ; Stastny, P. ; Jasin, H. E. / High levels of in vitro IgM rheumatoid factor synthesis correlate with HLA-DR4 in normal individuals. In: Arthritis and Rheumatism. 1987 ; Vol. 30, No. 8. pp. 841-848.
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